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Comprehensive Guide to Gram Staining and Microscopy in Clinical Microbiology

This document serves as a detailed answer key for Unit 1 of the Professional English for Clinical Microbiology course by Isabel Borja (NILE, 2011). It covers essential topics, including the significance of Gram staining, classifications of bacteria based on Gram reactions, and microscopy fundamentals. Activities designed to enhance understanding of bacterial shapes, arrangements, and culture media preparation are included. The information is invaluable for students and professionals in microbiology, facilitating effective learning and application in clinical settings.

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Comprehensive Guide to Gram Staining and Microscopy in Clinical Microbiology

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  1. CLIL Project Professional English for Clinical Microbiology Answer key Unit 1 Isabel Borja NILE 2011

  2. Answer key Lesson 1.1 – Gram Stain

  3. Activity 1 Significance of Gram stain: Gram stain is the main stain procedure in Microbiology. It enables us to know several things about bacteria in the smear: - GRAM-STAIN REACTION - SHAPE - ARRANGEMENTSof bacteria Bacteria are divided into two large groups depending on their Gram-stain reaction: the so-called Gram-positive (GP) that remains coloured after the decolouration procedure and the Gram-negative (GN) that do not retain the dye and take the colour of the counterstain. Those differences rely on the basis of cell wall structures. Considering together Gram reaction and shape we may set the four big groups of bacteria: GP bacilli, GP cocci, GN bacilli, and GN cocci.

  4. Activity 2

  5. Activity 3

  6. Activity 4

  7. Activity 5

  8. Answer key Lesson 1.2 – The Microscope

  9. Activity 1

  10. Activity 2.a EYEPIECE ARM NOSEPIECE OBJECTIVE STAGE CLIPS COARSE FOCUS KNOB STAGE CONDENSER DIAPHRAGM LEVER FINE FOCUS KNOB CONDENSER STAGE KNOBS BASE

  11. Activity 2.b

  12. Activity 3

  13. Activity 3

  14. Activity 3

  15. Activity 4

  16. Activity 5 Image 1 B: cerebrospinal fluid Image 4 E: sputum Image 2 C: urethral discharge Image 3 A: vaginal discharge Image 5 D: urine

  17. Answer key Lesson 1.3 – Preparing Culture Media

  18. Activity 1

  19. Activity 2

  20. Activity 2

  21. Activity 3

  22. Activity 4

  23. Activity 5

  24. Answer key Lesson 1.4 – Bacterial Culture

  25. Activity 1

  26. Activity 2

  27. Activity 3 Streak plate method: First spread Initial inoculum Signature Second spread Final spread

  28. Activity 4

  29. Activity 5 Activity 5.a - Bacterial standards:

  30. Activity 5

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