Chapter 23 (Part 1). Recombinant DNA Technology. Cloning Vector. Required features Origin of replication Selectable marker Screenable marker for recombinant molecules Cloning sites. AAGATGC GAATTC GTACA. AAGATGC GAATTC GTACA. DNA methylase. DNA methylase. *. *. AAGATGC GAATTC GTACA.
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Recombinant DNA Technology
3’ TACGCTTAAGGCCTT 5’
5’ ATGCGATATCCGGTT 3’
3’ TACGCTATAGGCCTT 5’
0.1 to 1% of cellular protein
10 to 70% of cellular protein
Adagen (Adenosine deaminase )
Severe combined immunodeficiency disease (SCID)
Genotropin (Recombinant growth hormone)
Growth hormone deficiency (GHD) in children
Pharmacia & Upjohn
Humalog (Recombinant human insulin)
Nabi-HB (Anti-Hepatitis B)
Novo Seven (Recombinant coagulation factor VIIa)
A modified factor
Hemophillia patients with inhibitors
Ontak (Diphtheria toxin-interleukin-2)
A fusion protein
Cutaneous T-cell lymphoma (CTCL)
Roferon-A (Recombinant interferon alfa-2a)
Hairy cell leukemia or AIDS-related Kaposi's sarcoma
Is Bigger Better?