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Understanding Fluorescence Peaks: Positive vs. Negative Medians and Robust Standard Deviation

Learn the difference between positive and negative peak medians, as well as the use of 2x rSD in fluorescence analysis. This guide by Dr. Holden Maecker explains concepts such as resolution sensitivity and fluorochrome-stain index.

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Understanding Fluorescence Peaks: Positive vs. Negative Medians and Robust Standard Deviation

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  1. W1 W2 Where D = difference between positive and negative peak medians, and W = 2 x rSD (robust standard deviation) “Bright” = good resolution sensitivity D Holden Maecker, Ph.D.

  2. Various fluorochromes-stain index

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