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Advanced Mass Spectrometry Techniques for Quantitative Proteomics Analysis

Explore the application of advanced mass spectrometry techniques such as iTRAQ and TMT for accurate quantitation in proteomics, using amine-specific peptide reactive groups like NHS for tagging. Results show a detailed analysis of multiple data sets with specific peptide sequences identified across different samples and instruments.

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Advanced Mass Spectrometry Techniques for Quantitative Proteomics Analysis

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  1. 13C 18O (+3) 18O (+2) 13C (+1) (+0) Balance group mass 31-28 (neutral loss) Reporter group mass 114-117 (retains charge) m/z 114 (+1) 13C m/z 115 (+2) 13C2 m/z 116 (+3) 13C215N m/z 117 (+4) 13C315N Quantitation by iTRAQ and TMT Amine specific peptide reactive group (NHS) Isobaric tag Total mass 144

  2. iTRAQ 8-plex

  3. Tandem Mass Tags (TMT)

  4. Participants 18 Data sets

  5. Results Not all protein data was submitted in the excel files. The total number of unique peptide sequences within each data set are 19207. Of these sequences 9468 are unique in all the data sets. # of peptides Only 3 peptides found in 15 data sets: 1 from CYC, 1 from MYG and the other from Ecoli. Data sets

  6. Results

  7. Results

  8. Results

  9. TMT Results MALDI TOFTOF Q TOF Orbitrap LTQ # of peptides Data Set

  10. Results

  11. TMT Results Mascot Phenyx ProteinPilot Sequest Quixote Ratio A/B Data Set

  12. TMT Results MALDI TOFTOF Q TOF Orbitrap LTQ Ratio A/B Data Set

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