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Mouthwash Effectiveness Against Oral Bacteria

Mouthwash Effectiveness Against Oral Bacteria. David A. Adams Microbiology Undergraduate Tennessee Technological University Cookeville, TN 38505. Introduction. Undesirable oral odors generally come from proteins and peptides hydrolyzed by gram-negative bacteria (McDowell 1993)

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Mouthwash Effectiveness Against Oral Bacteria

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  1. Mouthwash Effectiveness Against Oral Bacteria David A. Adams Microbiology Undergraduate Tennessee Technological University Cookeville, TN 38505

  2. Introduction • Undesirable oral odors generally come from proteins and peptides hydrolyzed by gram-negative bacteria (McDowell 1993) • Bacteria in the mouth are primarily responsible for gingivitis (Journal of American Dental Association 1998) • The number of viable bacteria dropped by 94% after rinsing with antiseptic mouth rinse (Fine et al 1993)

  3. Objective • The objective of this research was to determine the susceptibility of bacteria to different brands of mouthwash Hypothesis • Rinsing with mouthwash will have no benefit over rinsing with water to prevent bacterial growth.

  4. Methods & Materials • Methods were derived from Dr. Goss’ 2004 Medical Micro lab manual • 3 TSA plates inoculated with 3 different oral bacteria for 24 hours • Three individual mouthwashes (A, B, C) tested • Water used as control (D) • Performed three trials & took average

  5. B B C D C D A B C D A A Staphylococcus Escherichia Pseudomonas Methods & Materials Legend: A=Control B=Scope C=Listerine D=Equate

  6. Bacterial Inhibition Results

  7. Results

  8. Discussion • Staphylococcus was the least resistant bacteria • Pseudomonas and Escherichia were equally resistant • Better results may be obtained by testing a greater number of different oral bacteria

  9. Conclusion • All 3 mouthwash brands inhibited bacterial growth • The generic brand mouthwash, Equate, was the most effective • More cost does not always equal better value

  10. Questions?

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