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Consumer Products Study

Consumer Products Study. Disinfectants, Mouthwash Antibacterial Hand Soap, Antibiotics, and Spices. By THE WESTON SCHOLARS!!!!! (and gifted and talented students.). MATERIALS. Tape Wax pencil Petri dish Cotton swabs Forceps Nutrient broth Sterile disk Inoculating wire loop

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Consumer Products Study

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  1. Consumer Products Study Disinfectants, Mouthwash Antibacterial Hand Soap, Antibiotics, and Spices By THE WESTON SCHOLARS!!!!! (and gifted and talented students.)

  2. MATERIALS • Tape • Wax pencil • Petri dish • Cotton swabs • Forceps • Nutrient broth • Sterile disk • Inoculating wire loop • Sterile water • Bunsen burner • Nutrient agar plate • All consumer products

  3. Procedure Preparing Nutrient Agar Plates • Suspend 23g of nutrient agar powder in 1L purified H2O. • Mix and heat solution until powder is dissolved and solution becomes an translucent yellow. • Autoclave at 121ºC for 15 minutes. • Fill as many petri dishes as possible (half full) with nutrient agar and let them solidify.

  4. Procedure (cont’d) • Dip cotton swab in sterile H2O and take samples from mouth, skin, and environment. • Rub the swab on nutrient agar plate. • Cover nutrient agar plates and label them. • Invert nutrient agar plates and place them into the incubator at 37ºC.

  5. Procedure (cont’d) • For discontinuous streaking, sterilize wire loop by flaming until the wire turns bright orange. • Cool loop by lightly touching the wire to the nutrient agar. • Using the wire loop, take bacteria from the nutrient agar plate and streak 4 times on a new agar plate and flame the loop in between. • Cover, label, and tape plates shut. • Place, inverted, in the incubator at 37°C.

  6. Discontinuous Streaking

  7. Procedure (cont’d) • For the continuous streaking, use the same procedure as the discontinuous streaking, except you just streak back and forth until you cover the whole nutrient agar plate.

  8. Continuous Streaking

  9. Procedure (cont’d) Re-suspend bacteria 1. Pour 10ml of nutrient broth into the continuously streaked plates. 2. Swab bacteria until all bacteria is suspended in liquid 3. Swab bacteria into new plates. 4. Dip sterile disk in disinfectants, antibiotics, spices, mouthwash, or antibacterial hand soap and place in center of plates. Tap gently to secure. 5. Label plates and place them inverted in the incubator. 6. Let plates sit in the incubator over night. 7. Measure the zone of inhibition (in millimeters) and make your conclusions.

  10. Zone of Inhibition

  11. Disinfectants • Ajax • Algacide • America’s Choice Pine Cleaner • Ammonia • 10% Bleach • Clorox • Fantastik-Clear • Fantastik-Purple • Scrubbin’ Bubbles • Soft Scrub • Spic and Span • Tilex • Touch of Glass • Wavicide • White Vinegar • Windex • Xtra Pine • 409

  12. Disinfectants

  13. Results

  14. Results

  15. Best Disinfectants #1 #3 #2

  16. Mouthwashes • Biotene • Cepacol • CVS Brand • Diluted Hydrogen Peroxide • Listerine • Listerine Tartar Control • Plax • Rite Aid Brand • Salt Water • Scope • ShopRite Brand • Tom’s Natural Mouthwash

  17. Results

  18. Results

  19. Best Mouthwashes #1 #3 #2

  20. Hand Soaps • Bath & Body Works • Clean n Smooth • Ajax • Palmolive • Soft Soap • April Body & Shower

  21. Results

  22. Results

  23. Best Hand Soaps #3 #1 #2

  24. Antibiotics on the skin bacteria • Tetracycline • Rifampin • Chloramphenicol • Kanamycin • Streptomycin • Triple Sulfonides • Polymyxin B • Erythomycin • Cephalothin

  25. Results

  26. Results

  27. Best Antibiotics #1 Rifampin #2 Caphalothin #3 Chloramphenicol

  28. Spices • Cinnamon • Paprika • Chili Powder • Onion Powder • Cream of Tartar • Coriander • Mustard Powder • All Spice • Alum • Cumin • Nutmeg • Cajun Seasoning • Curry • Ground Ginger • Garam Marsala • Garlic

  29. Results

  30. Results

  31. Best Spices #3 #1 #2

  32. Thank you! Dr. Lee H. Lee Pat Platner Shi-Fang Hsu Tina Chu Alex Peroff Ms. English Ms. Portas AND Mr. & Mrs. Weston

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