Microscope Review. Give the name and function of each structure labeled. A – Ocular : eyepiece used to look at specimen B – Fine adjustment : to focus specimen under high power C – Arm : used to hold microscope D – Objective lens : used to magnify image
A– Ocular: eyepiece used to look at specimen
B – Fine adjustment: to focus specimen under high power
C – Arm: used to hold microscope
D – Objective lens: used to magnify image
E – Coarse adjustment: Used to focus specimen under low power
F – Diaphragm – adjust amount of light reaching specimen
Objective - Ocular
40 x 10 =
The amount of light decreases (so the field of view gets darker)
The field of view decreases ( so you see less of the slide).
The fine adjustment
because using the coarse adjustment under high power can break the objective lens or the slide.
Adjust the diaphragm to make the filed of view brighter.
Focus with the fine adjustment.
The number of cells that are visible in the field of view decreases.
Center the specimen
Focus with the coarse adjustment
Preparing a wet mount.
1. Place a drop of water on slide.
2. Place specimen on slide.
3. Place the cover slip at an angle.
4. Slowly lower the cover slip on to the specimen.
To avoid air bubbles.
The total magnification of an image is the result of the combined magnifications of the
(1) adjust the eyepiece
(2) center the image being viewed
(3) remove the slide from the stage
(4) remove the coverslip
(1) Its diameter will decrease.
(2) Its diameter will increase.
(3) Its brightness will increase.
(4) Its brightness will remain the same.
(1) 2,000 mm (2) 2 mm
(3) 5 mm (4) 500 mm
(1) nucleus (2) ribosome
(3) cell wall (4) mitochondrion
(1) field of vision is smaller under low power than under high power
(2) field of vision is larger under low power than under high power
(3) specimen does not need to be stained for observation under low power but must be stained for observation under high power
(4) amount of the specimen that can be observed under low power is less than the amount that can be observed under high power
(1) methylene blue
(2) dilute acid
(3) Benedict's solution
(4) bromthymol blue
(1) larger and brighter
(2) smaller and brighter
(3) larger and darker
(4) smaller and darker
(1) frog for dissection
(2) solution of iodine for food testing
(3) wet mount of elodea (a simple plant)
(4) test to determine the pH of a solution
Which part of a microscope should be used with the low power objective, but not with the high-power objective?
a. fine adjustment
c. coarse adjustment
Which image will the student most likely see?
A prepared slide was placed on the stage of a microscope so that the slide faced the student, as shown in the diagram below.
1) 250 um 2) 500 um 3) 1,000 um 4) 1,500 um
(1) A (2) B (3) C (4) D
The length of the cell is approximately 1750um.
Field of view = 1.5mm