1 / 11

Transcription in E.coli by the numbers

Explore transcription in E. coli through numbers - gene size, mRNA characteristics, production rates, degradation rates, and the genetic switch mechanism. Learn about Poisson distribution, single-cell trajectories, bursting transcription, and the dynamics of genetic regulation.

tasha
Download Presentation

Transcription in E.coli by the numbers

An Image/Link below is provided (as is) to download presentation Download Policy: Content on the Website is provided to you AS IS for your information and personal use and may not be sold / licensed / shared on other websites without getting consent from its author. Content is provided to you AS IS for your information and personal use only. Download presentation by click this link. While downloading, if for some reason you are not able to download a presentation, the publisher may have deleted the file from their server. During download, if you can't get a presentation, the file might be deleted by the publisher.

E N D

Presentation Transcript

  1. Transcription in E.coli by the numbers • Size of typical gene = 1kb • Typical number of mRNAs = 10 • Typical rate of mRNA production = 10 min-1 • Typical mRNA degradation rate = 1 min-1

  2. mRNA production in E.coli Golding et al. ‘05 r=1/7min °=ln2/50min

  3. Master equation for mRNA production Master equation for p(m,t): Poisson distribution with mean Steady state:

  4. Variance of the mRNA number var(m) = 4 <m> Poisson: var(m) = <m> Poisson Inconsistent with unregulated transcription model

  5. Single cell trajectories ¢n Transcription occurs in bursts!

  6. Genetic switch Gardner, Cantor and Collins ‘00

  7. Flipping the genetic switch Bistability!

  8. Nullclines and flow field

  9. Phase portrait of the genetic switch

  10. Repressilator Elowitz and Leibler ‘00

  11. The Poisson distribution What is the distribution of people per square? Histogram Poisson Probability people/unit area

More Related