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Effects of rIgG1 13B8.2 on Permeabilized and Non-Permeabilized Jurkat T Cells

This study investigates the impact of rIgG1 13B8.2 on Jurkat T cells, comparing non-permeabilized and permeabilized states under different treatment durations (10 min and 40 min). The analysis presents data on ceramide-positive cells after antibody treatment, employing inhibitors like FB1, Imi, and Myr to assess their influence on ceramide expression. Results indicate varying levels of events among treated cells, crucial for understanding antibody interactions in immune responses.

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Effects of rIgG1 13B8.2 on Permeabilized and Non-Permeabilized Jurkat T Cells

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  1. Fig.3 A 4h-treatment 10 min-treatment 40 Non-permeabilized Jurkat T cells + rIgG1 13B8.2 11% + rIgG1 13B8.2 Events 102 102 101 100 101 100 21% 0 Permeabilized Jurkat T cells 40 + rIgG1 13B8.2 + rIgG1 13B8.2 50% Events 73% 102 101 100 0 102 101 100 Anti-ceramide antibody B 50 40 30 Ceramide-positive Jurkat T cells (%) 20 10 0 Inhibitor - - FB1 FB1 Imi Imi Myr Myr rIgG1 13B8.2 - + - + - + - +

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