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省產石花菜萃取物對細胞生長抑制的探討

省產石花菜萃取物對細胞生長抑制的探討. 中文摘要

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省產石花菜萃取物對細胞生長抑制的探討

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  1. 省產石花菜萃取物對細胞生長抑制的探討 • 中文摘要 • 本研究之目的乃在探討省產石花對於細胞之生長是否具有抑制作用，並且觀察其生長抑制之作用是否具有選擇性，同時並探討其是否因誘導細胞程序化凋亡(apoptosis)之故。石花菜分別以PBS及methanol萃取之，並依照一般食用方式製備石花凍，經冷凍乾燥後溶解於DMSO中而後偵測不同之萃取物對Hepa1(Murine hepatoma cell)、HL-60 Human promyelocytic leukemia cell) 二株癌細胞以及正常NIH3T3 Murine embryo fibroblast cell) 細胞生長的影響。細胞數目使用Coulter counter計算之，細胞增殖測試則使用MTS assay kit偵測，並以DNA電泳以及Annexin V-FITC螢光染色法觀察石花菜萃取物是否可誘導細胞產生apoptosis的現象。結果顯示，石花菜之PBS- soluble extract對3種細胞株之生長及增殖都沒有抑制的效果( p>0.05) ；但其methanol-soluble extract對Hepa1及NIH3T3細胞之生長及增殖有抑制的作用 (p<0.05)；且DMSO-soluble extract可抑制3種細胞株之生長及增殖的作用 (p<0.05)。螢光染色及DNA電泳分析結果顯示，Hepa1及NIH3T3細胞經methanol-soluble extract處理，3種細胞株經DMSO-soluble extract處理後，都有annexin V positive反應，細胞之DNA亦有斷片之產生，顯示石花菜萃取物會誘導細胞apoptosis的發生。總言之，石花菜之methanol- 及DMSO-soluble extract具有抑制細胞生長的果，且其生長抑制作用可能由於其可誘導細胞apoptosis的發生，但是此作用並不具有選擇性。

  2. Antiproliferation effects of Gelidium amansii extracts on cultured cells • 英文摘要 • The objective of this study was to investigate the effect of Gelidium amansii on the cell growth and the potential role of apoptosis on the growth inhibition was also explored. Two lines of cancer cells, murine hepatoma cell (Hepa1) and human promeyolitic leukemia cell (HL-60), and one normal cell lines, murine embryo fibroblast cell (NIH3T3) were used in this study. The cells were treated with PBS-, methanol-, or DMSO extracts of Gelidium amansii, and the cell growth and the cell proliferation were measured. The results indicate the PBS - soluble extract did not have the effects on the all lines of cells, whereas methanol-soluble extract had the inhibitory effect on the Hepa1 and NIH3T3 cells (p<0.05), and DMSO- soluble extract had inhibitory effects on all lines cells (p<0.05). The methanol-soluble extract treated Hepa1 and NIH3T3 cells, and all lines of cells treated with DMSO-soluble fraction of the Gelidium amansii showed annexin V positive response, and had fragmented DNA ladders on agarose gels indicating that the cells underwent apoptosis. Gelidium amansii possesse the antiproliferation effect of cultured cells and this may due to the induction of the apoptosis pathway, this inhibitory effect did not show cell specificity.

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