RNA, Protein Mitosis, Cytokinesis Lamin H1 Abl Cyc B/A CDK1 M Cyc D’s CDK4,6 G2 G0 1 h 3-4 h G1 DNA, RNA, Protein S RNA, Protein 6-12 h 6-8 h p53 pRb Cyc A CDK2 Cyc E CDK2 Cell Cycle Eric Niederhoffer SIU-SOM
Cdk4 Cdk2 Cdk1 D E A B(A) M G1 S G2 M G1 Start Variation in Cell Cycle Cyclins Cyclin-dependent kinases cyclins Cell cycle phases
Active p53 p21 P pRb Passage from G1 to S Cell Cycle Regulation 1. CDK phosphorylation DNA damage 2. C degradation 3. C & CDK synthesis CDK2 4. CDK inhibition CE pRb pRb pRb E2F Enzymes for DNA synthesis
Review Questions • What are and what happens during the phases of the cell cycle? • Which proteins are involved in the regulation of the cell cycle? • Which cyclins and cyclin-dependent kinases are most important in individual phases of the cell cycle? • What are four mechanisms for regulating cyclin-dependent kinase activity? • What role do p53, p21, and pRb play in the G1 to S transition?