slide1 n.
Download
Skip this Video
Loading SlideShow in 5 Seconds..
PowerPoint Presentation
Download Presentation

Loading in 2 Seconds...

play fullscreen
1 / 1

- PowerPoint PPT Presentation


  • 133 Views
  • Uploaded on

A. B. C. D.

loader
I am the owner, or an agent authorized to act on behalf of the owner, of the copyrighted work described.
capcha
Download Presentation

PowerPoint Slideshow about '' - tania


An Image/Link below is provided (as is) to download presentation

Download Policy: Content on the Website is provided to you AS IS for your information and personal use and may not be sold / licensed / shared on other websites without getting consent from its author.While downloading, if for some reason you are not able to download a presentation, the publisher may have deleted the file from their server.


- - - - - - - - - - - - - - - - - - - - - - - - - - E N D - - - - - - - - - - - - - - - - - - - - - - - - - -
Presentation Transcript
slide1

A

B

C

D

Additional file 4: Effect of cysteineproteaseinhibitor E-64C on CcCP4 activity. The inhibitoryeffect of the cysteineproteaseinhibitor E-64C on the activity of CcCP4 wastested as follows: 10µL (3.2µg) His-tag purified and dialysed recombinant HIS-SUMO-CP4 protease was added to 20µl sodium formate (pH3) and incubated 30 second in a 37°C water-bath. Then, either 100µM E-64C (Panel A), or 10µM E-64C (Panel B), or 1µM E-64C (Panel C), or no E-64C (Panel D, control), wereadded, immediatelyfollowed by the addition of 6.7µL BSA reaction buffer (seemethods section). For eachreaction, 3 ulsamplesweretakenat the start (T=0), and at T=5min, T=10min, T=3h, T=4h30, and immediatelyadded to 5 ul 5x SDS gel loading buffer. The samplesweresubsequentlyrun on 8-16% SDS-PAGE gels and stained by coomassie. Lane M, Molecular marker with the sizes shown on the left in kDa (BioradPrecision Protein™ Standards, prestained).