Paper Chromatography. Identifying the components of a mixture. Chromatography. Chromatography is a method for separating mixtures into their components based on physical and/or chemical properties of the components.
Download Policy: Content on the Website is provided to you AS IS for your information and personal use and may not be sold / licensed / shared on other websites without getting consent from its author.While downloading, if for some reason you are not able to download a presentation, the publisher may have deleted the file from their server.
Identifying the components of a mixture
Chromatography is a method for separating mixtures into their components based on physical and/or chemical properties of the components.
Developed around 1903 by Russian Mikhail Semenovich Tswett in which he separated plant pigments on “diatomaceous earth” with alcohol
Rf ratio of spot distance to solvent front distance
Put the spotted paper in a developing tank
Solubility – the degree to which a material (solute) dissolves into a solvent. Solutes dissolve into solvents that have similar properties. This allows different solutes to be separated by different combinations of solvents.
Separation of components depends on both their solubility in the mobile phase and their differential affinity to the mobile phase and the stationary phase.Principles of Paper Chromatography