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Valorization of glycerol through the production of biopolymers: The PHB case using Bacillus megaterium. Javier M. Naranjo , John A. Posada, Juan C. Higuita , Carlos A. Cardona Instituto de Biotecnología y Agroindustria, Universidad Nacional de Colombia sede Manizales, .

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slide1

Valorization of glycerol through the production of biopolymers: The PHB case using Bacillus megaterium

Javier M. Naranjo, John A. Posada, Juan C. Higuita, Carlos A. Cardona

Instituto de Biotecnología y Agroindustria, Universidad Nacional de Colombia sede Manizales,

Betreuer: Benjamen Stenger

Ammar Abdulmughni

overview
Overview
  • Introduction

Polyhydroxyakanoate (PHA):

Structure, Biosynthesis

Production

  • Aimofthissubject
  • Results
  • Summary
polyhydroxyakanoate pha
Polyhydroxyakanoate (PHA)
  • PHAs are linear polyesters
  • Manyprokaryotescansynthesizeandstore PHAs in form ofgranules (upto 80 % of total bacterialweight)
  • PHAs havesimilarmechanicalpropertiestoconventionalplastics
  • Theyaregenerallybiodegradable
polyhydroxyakanoate pha1
Polyhydroxyakanoate (PHA)
  • The biosynthesis of PHA :

certain deficiency conditions and

excess supply of carbon sources

  • The mostcommon/produced form of PHA ispolyhydroxybutyrate, PHB
  • Likepolypropyleneorpolyethylene.

PHB

phb production
PHB Production
  • Productioncostsareheigh:

 PHB accumulateintercellularly:

- Useof a solvent

- thermal treatment of biomass, enzymatic digestion and washing with an

surfactant

a self-disruptivestrain.

slide7
The lysissystemofBacillusamyloliquefaciens
  • phage was insertedintoshuttlevector
  • The expression of a target gene is inhibited by
  • glucose
  •  When the glucose concentration approached
  • zero, self-disruption is induced
phb production1
PHB Production
  • Productioncostsareheigh:

carbonsources

The carbon source could account for 25–45% of the total production costs

Cheapercarbonsources

phb production2
PHB Production
  • Agroindustrial wastesareattractivecandidatesassubstrates:

 lowpricesandheighavailability

 solving an environmental problem

  • A variety of microorganisms are able to produce PHB from diverse agroindustrial wastes:

Methylobacteriumrhodesianum(Borman et al., 1999)

Cupriavidusnecator(Cavalheiro et al., 2009)

 E. coli CT1061 (Nikelet al., 2008)

glycerol is carbon source
Glyceroliscarbonsource
  • Crudeglycerolis a co-product in theproductionofbiodiesel
  • Glycerolis an importantindustrialfeedstock :

- food, drugs, cosmetics, pharmaceuticals, textile andtobaccoindustries

  • Glycerolcanbeusedascarbonsource in microbiologicalprocesses
  • Polyhydroxyakanoate (PHA) productionis an interestingbiologicaltransformationofglycerol.
phb production3
PHB Production
  • Currently, PHB is produced at an industrial scale using Gram negative bacteria

 lipopolysaccharides (LPS) which co-purify with the PHAs and induce a strong immunogenic reaction (Valappil et al., 2007)

Bacillusmegaterium

slide12
Aim
  • Techno-economicanalysisof PHB production:

- Glyceroltransformationinto PHB (usingBacillusmegaterium)

- Glycerolor Glucose assubstrate

- Productivityat different conditions

results
Results
  • Different initialconcentrationofglycerol

PHB Production

Biomass

The fermentation conditions:

temperature 30 °C, air flow 12 l/min and uncontrolled pH

results1
Results
  • Different temperaturesoffermentation

PHB Production

Biomass

The fermentation conditions:

initial glycerol concentration of 20 g/l, air flow 12 l/min, and uncontrolled pH

results2
Results
  • PHB productionusingglucoseorglycerol

VerySimilaryields

results3
Results
  • Batch cultivation:

The fermentations to produce PHB were carried out for 42 h in a 3.7 l Lab Fermen

results4
Results
  • Economicanalysis:
  • The current sale prices are between 3.1 and 4.4 USD/kg
summary
Summary
  • PHAs are linear polyestersandcanbesynthesizedanditnracellularlystoredbymanyprokaryotes.
  • The mostproduced form of PHA is PHB
  • PHAs havesimilarmechanicalpropertiestoconventionalplastics
  • Productioncostsareheigh
  • PHB ProductionusingB. megaterium, andglycerolascarbonsource
  • The results confirm the ability of B. megaterium to use glycerol as the only carbon source.
references
References
  • Valorization of glycerol through the production of biopolymers: The PHB caseusingBacillusmegaterium, Javier M. Naranjo, John A. Posada, Juan C. Higuita, Carlos A. Cardona, Bioresource Technology 133 (2013) 38–44.
  • Polyhydroxybutyratesynthesis on biodieselwastewaterusingmixedmicrobialconsortia, Zachary T. Dobroth a, Shengjun Hub, Erik R. Coats a, Armando G. McDonaldBioresource Technology 102 (2011) 3352–3359
  • Large-scale production and efficient recovery of PHB with desirable material properties, from the newly characterised Bacillus cereus SPV, Valappil SP, Misra SK, Boccaccini AR, Keshavarz T, Bucke C, Roy I., J Biotechnol. 2007 Nov 1;132(3):251-8
  • Construction of self-disruptive Bacillus megaterium in response to substrate exhaustion for polyhydroxybutyrate production. Hori K, Kaneko M, Tanji Y, Xing XH, Unno H. Graduate School of Bioscience and Biotechnology, 4259 Nagatsuta-cho, Midori-ku, Yokohama 226-8501, Japan.
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