1 / 9

添加文本

添加文本. 埃博霉素底盘的构建. 报告人：张洁. 埃博霉素在生物体中的表达. ① Science.2000. Tang et al . Cloning and heterologous expression of the epothilone gene cluster ② Antimicrob Agents and chemotherapy 2002. Julien and Shah. Heterologous Expression of Epothilone Biosynthetic Genes in Myxococcus Xanthus

taji
Download Presentation

添加文本

An Image/Link below is provided (as is) to download presentation Download Policy: Content on the Website is provided to you AS IS for your information and personal use and may not be sold / licensed / shared on other websites without getting consent from its author. Content is provided to you AS IS for your information and personal use only. Download presentation by click this link. While downloading, if for some reason you are not able to download a presentation, the publisher may have deleted the file from their server. During download, if you can't get a presentation, the file might be deleted by the publisher.

E N D

Presentation Transcript

  1. 添加文本 埃博霉素底盘的构建 报告人：张洁

  2. 埃博霉素在生物体中的表达 ① Science.2000. Tang et al . Cloning and heterologous expression of the epothilone gene cluster ②Antimicrob Agents and chemotherapy 2002. Julien and Shah. Heterologous Expression of Epothilone Biosynthetic Genes in Myxococcus Xanthus ③Biochmistry.2006. Mutka et al Heterologous production of epothilone C and D in E.coli ④Appl Microbiol Biotechnol.2008.Park et al Heterologous prodution of epothilone D in Streptomyces Venezuelae

  3. 选取底盘： 肉桂地链霉菌 丙二酰辅酶A 5个乙酸单位 1个丁酸单位 7个丙酸单位 甲基丙二酰辅酶A

  4. Organization of the monensin biosynthetic gene cluster of Streptomyces cinnamonensis ATCC15413

  5. 对Apr、Hyg完全抗，对Kan、neo部分抗 肉桂地链霉菌 游离型质粒整合型质粒 筛选高产菌株，莫能菌素产量稳定在7g/L左右

  6. 对底盘细胞的改造

  7. 分析底盘细胞中启动子的强弱 用报告基因法比较启动子的强弱

  8. EpoB EpoF EpoK EpoA EpoC EpoD EpoE MON MonH MonRⅡ MonRⅠ

  9. Thank You !

More Related