1 / 2

PCR Polymerase Chain Reaction

PCR Polymerase Chain Reaction. a tool to generate a large amount of a gene of interest. DNA-sequence in a plasmid. DNA. . . Restriction of plasmid releases the DNA sequence of interest. Plasmid. P olymerase C hain R eaction

Download Presentation

PCR Polymerase Chain Reaction

An Image/Link below is provided (as is) to download presentation Download Policy: Content on the Website is provided to you AS IS for your information and personal use and may not be sold / licensed / shared on other websites without getting consent from its author. Content is provided to you AS IS for your information and personal use only. Download presentation by click this link. While downloading, if for some reason you are not able to download a presentation, the publisher may have deleted the file from their server. During download, if you can't get a presentation, the file might be deleted by the publisher.

E N D

Presentation Transcript


  1. PCR Polymerase Chain Reaction a tool to generate a large amount of a gene of interest

  2. DNA-sequence in a plasmid DNA   Restriction of plasmid releases the DNA sequence of interest Plasmid Polymerase Chain Reaction synthesizes only the gene of interest by using Forward and Reverse primers (specific small pieces of DNA) with the help of the enzyme Polymerase. All cycles follow a strict order of timed temperatures. First cDNA strand Second cDNA strand Forward primer Reverse primer Gene of Interest

More Related