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Mastering PCR: Your Gene Synthesis Tool

Utilize PCR to amplify specific DNA sequences in plasmids, enabling gene synthesis. Learn about primer design, enzyme Polymerase, and temperature cycles for efficient gene amplification.

sydney-ayers
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Mastering PCR: Your Gene Synthesis Tool

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  1. PCR Polymerase Chain Reaction a tool to generate a large amount of a gene of interest

  2. DNA-sequence in a plasmid DNA   Restriction of plasmid releases the DNA sequence of interest Plasmid Polymerase Chain Reaction synthesizes only the gene of interest by using Forward and Reverse primers (specific small pieces of DNA) with the help of the enzyme Polymerase. All cycles follow a strict order of timed temperatures. First cDNA strand Second cDNA strand Forward primer Reverse primer Gene of Interest

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