1 / 2

Results/Summary : 58 of 208 blood samples (28 %) from 33 patients contained DTC

enrichment of DTC in peripheral blood samples of 101 patients with transitional cell carcinoma of the bladder (bTCC); 208 blood samples (BS) before & after TUR-B, cystectomy (cys) density gradient centrifugation (Ficoll) by magnetic cells sorting (AutoMACS, Miltenyi Biotec)

sutton
Download Presentation

Results/Summary : 58 of 208 blood samples (28 %) from 33 patients contained DTC

An Image/Link below is provided (as is) to download presentation Download Policy: Content on the Website is provided to you AS IS for your information and personal use and may not be sold / licensed / shared on other websites without getting consent from its author. Content is provided to you AS IS for your information and personal use only. Download presentation by click this link. While downloading, if for some reason you are not able to download a presentation, the publisher may have deleted the file from their server. During download, if you can't get a presentation, the file might be deleted by the publisher.

E N D

Presentation Transcript

  1. enrichment of DTC in peripheral blood samples of 101 patients with transitional cell carcinoma of the bladder (bTCC); • 208 blood samples(BS)before & after TUR-B, cystectomy (cys) • density gradient centrifugation (Ficoll) by magnetic cells sorting • (AutoMACS, Miltenyi Biotec) • depletion of leukocytes (CD45 microbeads) with • DTC in the negative fraction • Cytospin centrifugation and ICC (anti-CK-ab MNF116, Dako) • DTC detection & quantification by positive CK staining and patho-morphological assessment, FISH (UroVysion) for selected cases • detection of typical chromosomal alterations Detection of disseminated tumor cells (DTC) in the peripheral blood of bladder cancer patients for therapy assessment U. Schmidt1, A. Meye1Udo Bilkenroth2, Katja Robel1, S. Fuessel1, Clemens Linné1, Christian Höfling1, Oliver W. Hakenberg1, M.P. Wirth1 1Department of Urology, Technical University of Dresden, Germany; 2Department of Pathology, Martin-Luther-University of Halle, Germany

  2. Results/Summary: • 58 of 208 blood samples (28 %) from 33 patients contained DTC • detection of DTC independent of tumor stage & grade • cells of bTCC disseminate early • less DTC after TUR-B & cystectomy compared to pre-operative BS • surgical intervention caused a clear reduction of DTC Detection of disseminated tumor cells (DTC) in the peripheral blood of bladder cancer patients for therapy assessment

More Related