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Castel_Figure S1, related to Figure 1

Castel_Figure S1, related to Figure 1. RT-qPCR: Hey1. A. B. Relative mRNA levels (2^- DD Ct ). Sensitivity. GimmeMotifs. Transfac M00234. 1-Specificity. C. D. ChIP: Ab1-RBPJ. WT. Rbpj —/—. % ChIP /input. Krt9. Hey1. neg16. Rbms1. Rps6ka1. Apobec3. Atp6v0a4.

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Castel_Figure S1, related to Figure 1

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  1. Castel_Figure S1, related to Figure 1 RT-qPCR: Hey1 A B Relative mRNAlevels (2^-DDCt) Sensitivity GimmeMotifs Transfac M00234 1-Specificity C D ChIP: Ab1-RBPJ WT Rbpj—/— %ChIP/input Krt9 Hey1 neg16 Rbms1 Rps6ka1 Apobec3 Atp6v0a4 RBPJPhalloidinHoechst Genes associated to peaks E F

  2. Castel_Figure S2, related to Figure 2 C 24 hours Notch: On Off On Off RBPJ p300 Inducible Constant D Replicate experiment ChIP:Ab1-RBPJ 24 hours 6 hours Notch: On Off On Off Inducible Constant 6 hours 24 hours RBPJ RBPJ Notch: On Off On Off Inducible Constant

  3. Castel_Figure S3 A Validation with an independent RBPJ polyclonal antibody (Ab2-RBPJ) Notch: On Off On Off Inducible Ab2-RBPJ Constant Ab1-RBPJ Ab1-RBPJ Ab2-RBPJ B Notch: On On Off Off Inducible Constant

  4. Castel_Figure S3 C REST motif in Ab2-RBPJ only sites RBPJ (GM) RBPJ (M00234) REST CREB ETS Frequency D Ab1-RBPJ sites Ab2-RBPJ only sites Sensitivity 1-Specificity 1-Specificity E F ChIP on REST sites ChIP: Ab2-RBPJ %ChIP/input %ChIP/input peaks: Krt9 Hey1 neg16 Rbms1 Rps6ka1 Apobec3 Atp6v0a4 ChIP: Ab1-RBPJ ChIP: Ab2-RBPJ Genes associated to peaks

  5. Castel_Figure S4, related to Figure 3 A B NICD-GFP GFP 49kD HEYL 38kD C 28kD RBPJ/NICD-GFP NICD-GFP only RBPJ only Constant RBPJ only Inducible frequency frequency frequency frequency position (bp) position (bp) position (bp) position (bp) D E

  6. Castel_Figure S4, related to Figure 3 F

  7. Castel_Figure S5, related to Figure 4 A B C

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