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This study investigates the effects of CPS on Fas/CD95 expression and TRPV1 clustering in RT4 cells. Using confocal microscopy, we examined the immunocytochemical localization of Fas/CD95 and TRPV1 after treating the cells for 24 hours with 100 μM of CPS, with or without 10 μM of CPZ. The results indicate a significant increase in Fas/CD95 expression in a TRPV1-dependent manner, suggesting potential implications for understanding urothelial and glioma cell response to CPS. The findings contribute to the broader research on transient receptor potential channels.
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Fig. 49.1 CPS increases Fas/CD95 expression and clustering in TRPV1-dependent manner in RT4 cells. The immunocytochemical localization of Fas/CD95 and TRPV1 in RT4 cells treated for 24 h with 100 μM of CPS alone or in combination with 10 μM of CPZ was analyzed by confocal microscopy using an anti-Fas/CD95 mAb and a goat anti-TRPV1 Ab followed by respective secondary Abs. Control sample indicates DMSO vehicle Transient Receptor Potential Channels, Md. Shahidul Islam (Ed.) ISBN: 978-94-007-0264-6, Springer
Fig. 49.2 TRPV mRNA expression as determined by reverse transcription–PCR. (a) TRPVs mRNA expression in urothelial carcinoma cell lines. (b) TRPVs mRNA expression in glioma cell lines Transient Receptor Potential Channels, Md. Shahidul Islam (Ed.) ISBN: 978-94-007-0264-6, Springer
