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Water structures carrying DNA information, Application to HIV/AIDS and Autism. L.Montagnier, Borovets, October 23, 2013. The Evolution of Medicine Toward 4 P Qualities P1 Preventive P2 Predictive P3 Personalized P4 Participative. Emergence or reemergence of new epidemics due to :

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Water structures carrying DNA information, Application to HIV/AIDS and Autism


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    1. Water structures carrying DNA information, Application to HIV/AIDS and Autism L.Montagnier, Borovets, October 23, 2013

    2. The Evolution of Medicine Toward 4 P Qualities P1 Preventive P2 Predictive P3 Personalized P4 Participative

    3. Emergence or reemergence • of new epidemics due to : • Globalization of exchanges and travels • Demography: concentration in large cities • Nutrition (pesticides, water) • Environmental factors • Climate changes, electromagnetic radiations • Contacts with wild and farm animals • Decline of immune defenses

    4. - Cancers- Cardiovascular- Neurodegenerative- Arthritic- Autoimmune- Multifactorial, but in common: oxidative stress infectious agents (?) The most important burdenChronic Diseases

    5. Radiations (, X, UV, visible, hertzian) Air chemical pollution Inadequate Food Excessive physical exercize tobacco smoke alcohol ischemia Parasitic infections Bacterial infections Viral infections Various environmental factors effects accumulate + + + + + + + + + = DISEASE

    6. Lipid hyperoxydation (plasma, cell membranes) Protein Oxydation --> destruction, agregates DNA oxydation --> Mutations, Chromosomal Breakage Pathological effects of Oxydative Stress

    7. Oxidative stress Weakens the immune system Activates transcription factors (NF-kappa B) Activates genes involved in cell division, inflammatory cytokines, lymphocytes activation Immune dysfunction, apoptosis (TH1 TH2)

    8. The Human Genome Genes (+ introns +promoters) 3% Retroelements: retroviruses 8% retrotransposons 13% LTR 23% Total 47% Repeats 50% LINE 22% SINE 13% Unknown ?

    9. MucosaSkin Our microbial ecosystem : Constant exposure to microbial agents and immune protection

    10. Extreme genetic plasticity • of microorganisms: • virus : HIV, Influenza • Bacteria (plasmids) • Parasites (retrotransposons) • against reaction of the immune system

    11. - Biofilms- Mobile antibiotic resistant genes- Nanoforms- Nanostructures- Endosymbiontes How bacteria have learned how to persist despite the immune system

    12. Parasite Bacterium Virus (Worm) intracellular Filarial nematode Wolbachia

    13. Persistent cold infections • Antibiotics affect them only when they go out of sanctuaries • Inability of the immune system to eradicate them • (tolerance, oxidative stress) • Non-multiplicative forms of « classical » bacteria • intracellular bacteria • Sanctuaries • (bone marrow, joints, intestine, brain, ….) • Vectors (Parasites) • Remote effects (toxin, nanostructures)

    14. EXPLORING THE ROLE OF LATENT INFECTION IN CHRONIC DISEASES A physical and molecular approach

    15. Two technologies for detecting bacteria and viral DNA’s • A new technology based on the production of electromagnetic waves • The Polymerase Chain Reaction (PCR) using the 16S ribosomal DNA variability

    16. Classical model of PCR oligo Taq polymerase oligo

    17. Resonance emission of low frequency electromagnetic waves by high water dilutions of DNA. A newly discovered property of DNA :

    18. Molecular recognition without close contact (by waves and resonance)

    19. Capture of the signals Signal Analysis software Sample X 500 Sensor coil Computer Amplifier

    20. Detection of Ultra Low Frequencies Waves (ULF 500-2000 hertz) in certain dilutions of filtrates (100nM, 20nM, 15nM) from cultures of micro-organisms (virus, bacteria) or from the plasma of humans infected with the same agents. FACTS

    21. 7-100 Hz Filtration 0.1 0.02 2ng/1ml 10-2 10-3 10-4 10-5 10-6 10-7 10-8 10-9 10-10 10-12 10-13 10-14 10-15 10-16 1000 - 3000 Hz

    22. Noise Amplitude (+) Time (sec)

    23. Spectral Frequency Analysis Fourier Tranformation • A positive signal is defined by: • amplitude increase • Shift to higher frequencies (500-2000 Hertz)

    24. Noise (+) Time (sec) Frequency (1-20000 Hertz)

    25. Suspensions of pure culture ofbacteria with pathogenic potentialare producing electromagnetic signals (EMS) in the range of dilution usually 10-810-13, sometime (E.coli) up to 10-18 They are:E.coli, Streptococcus, Salmonella, Staphylococcus, B.subtilis, Clostridium, Pseudomonas, etc.

    26. Micro-organisms involved in EMS induction 1.DNA from main pathogenic bacteria Streptococcus Staphylococcus Pseudomonas Mycoplasma pirum Salmonella Clostridium Proteus mirabilis B. Subtilis Borrelia burgdorferi Sutterella - From viruses HIV1 Influenza group A HBV HCV - Genes involved M.pirum adhesin HIV genes

    27. 10-2 Water excitated state crystal like gel no EMS DNA Interpretation 10-9 Free polymer self maintained by EMS

    28. I – DNA’s emit EMS II – EMS are produced by water nanostructures (naneons) III – EMS are producing naneons IV – Naneons and EMS carry specific DNA information

    29. EMS are produced by water • nanostructures (naneons) • Evidence : • from filtration • Size : between 20 and 100 nM for bacterial sequences • Smaller that 20µM for viral sequences • from biophysical studies • indicating spectrometral changes in the dilutions • producing EMS

    30. Naneons and EMS carry specific DNA information Natural and digital transmission

    31. Classical model of PCR oligo Taq polymerase oligo

    32. PCR on water nanostructures oligo Taq EMS oligo

    33. Water Naneons DNA EMS EMS Water Naneons PCR DNA 7 Hz

    34. Generator 7Hz DNA -6 -6 water Tube 1 Tube 2 µmetal -2 -3 -4 -5 -6 18hrs water + + + EMS

    35. D-4 LTR HIV DNA (104bp) 7Hz, 18 Hrs and then PCR (35 cycles) from D-2 to D-15 after filtration 450 and 20 nM Transmission in water of D-4 LTR HIV DNA (104bp) 7Hz, 18 Hrs and then PCR (35 cycles) from D-2 to D-15 after filtration 450 and 20 nM DW: Distilled Water / FD2: Dilution 10-2 after filtration 450and 20 nM

    36. Water-mediated photonic transmission of DNA Water Naneons DNA Computer Digitized EMS Receiver Computer PCR EMS Analog DNA Water Naneons

    37. Reproduction of DNA transduction in other laboratories File EMS of 194 bp DNA from HIV1 LTR Sent to Benevento University, Molecular Biology Laboratory DNA reproduced and sequenced 100 % identical to original File EMS of 499 bp DNA from Borrelia burgdorferi Sent to Laboratory of Chronix Biomedicals University of Gottingen

    38. Reproduction of DNA transduction in other laboratories File EMS of 194 bp DNA from HIV1 LTR Sent to Benevento University, Molecular Biology Laboratory DNA reproduced and sequenced 100 % identical to original File EMS of 499 bp DNA from Borrelia burgdorferi Sent to Laboratory of Chronix Biomedicals University of Gottingen

    39. Water-mediated photonic transmission of DNA Gel electrophoresis of the PCR DNA product (Borrelia Burgdorferi) E.Schutz et al. Goettingen, 2011

    40. ELF DNA DNA DNA WATER DNA (plasma) DNA How pathogenic information can persist, and escape immune defence and treatment.

    41. Two main problems for AIDS research in 2013 - The viral DNA reservoir- The Higher sensitivity of African people to HIV infection

    42. The dual origin of EMS in HIV/AIDS EMS plasma RBC HIV < 20 nM ++ +/- Agent X< 100 nM - ++

    43. Evidence that the agent X is a bacterium • Presence of a sequence close to 16 S ribosomal DNA • of Rickettsiales • Sensitive to antibiotics ( in vitro and in vivo) • Growth in cell line • Electron microscopy • Associated with Red Blood Cells