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This supplemental figure (Supplemental Figure 2) presents the quantification of phosphorylated STAT5 (pSTAT5) levels in various cell lines expressing mutations A.D816V and B.D816V. The experimental setup includes GST-STAT5 constructs and involves measuring pSTAT5 levels using specific assays. The data demonstrate how these mutations influence pSTAT5 activation, essential for understanding their role in cellular signaling and oncogenesis. Detailed methodologies and results are provided within the figure for in-depth analysis.
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A D816V Y568-Y570FF B D816V Y568-Y570FF GST-STAT5-Y (ng) 0 200 400 0 200 400 GST-STAT5 Y F Y F pSTAT5 pSTAT5 KIT GST Quantification of pSTAT5 C Supplemental Figure 2