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Characterization of Coenzyme Q10-loaded Ethyl Cellulose Nanoparticles using HPLC, Dynamic Light Scattering, Electron M

Characterization of Coenzyme Q10-loaded Ethyl Cellulose Nanoparticles using HPLC, Dynamic Light Scattering, Electron Microscopy and DSC. Coenzyme Q10. a nutraceutical ( substance with associated heath benefits ) an antioxidant. from www.sigmaaldrich.com. Ethyl cellulose.

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Characterization of Coenzyme Q10-loaded Ethyl Cellulose Nanoparticles using HPLC, Dynamic Light Scattering, Electron M

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  1. Characterization of Coenzyme Q10-loaded Ethyl Cellulose Nanoparticles using HPLC, Dynamic Light Scattering, Electron Microscopy and DSC

  2. Coenzyme Q10 • a nutraceutical (substance with associated heath benefits) • an antioxidant from www.sigmaaldrich.com

  3. Ethyl cellulose • a biomaterial used for microencapsulation • derived from cellulose and ethanol in the presence of dehydrating agents OR alkali cellulose and ethyl chloride from www.sigmaaldrich.com

  4. Nanoencapsulation of coQ10 Nanoencapsulation • separates individual molecules w/o altering its function • Increases bioavailability in the intestine CoQ10 tends to bunch together, making it hard to absorb in the intestine.

  5. Objective • Particle size • % drug loading • UV stability • thermal stability To determine physical properties of coenzyme Q10-loaded ethyl cellulose nanoparticles:

  6. Presupposition from Literature:Nanoparticles of ethyl cellulose can be prepared by solvent-evaporation Langmuir, 2003, 19(22), 9504-9510.

  7. Presupposition from Literature: Ethyl cellulose (EC) is used as a biomaterial media for encapsulation SEM images of EC microspheres For controlled release of a pesticide, norfluazon [Pest Management Science, 2001, 57: 688-694] For microencapsulation of aspirin, for drug delivery application [J. of Microencapsulation, 2001,18(2), 223-236]

  8. Presupposition from Literature: Coenzyme Q10 - loaded PMMAwere characterized usingTEM, Dynamic light scattering,DSC, HPLC, NMRto determine % drug loading Particle size Thermal stability Colloids and Surfaces A : Physicochemical and Engineering Aspects, 2002, 210 (1): 95-104.

  9. Presupposition from Literature: HPLC is a reliable method for quantitative analysis of CoQ10 coQ10 gives Absorbance at 217-290 nm Solvents: methanol, ethanol, water ( mobile phase) Column: C18 from www.boomer.org

  10. Methods • Prepare nanoparticles by solvent-evaporation method by Desgouilles [Langmuir, 2003,19(22), 9504-9510] Vary the mass ratio of coQ10 to EC (20, 40, 50, 60, 80%) • Characterization of nanoparticles

  11. Measurement of Particle Size Distribution by Dynamic Light Scattering scattering angle: 90° Temperature: 25 ° Hydrodynamic mean diameter and polydispersity, μ2/Γ2, will be calculated by continuous method Light Scattering facility at UNC Chapel Hill

  12. by Scanning Transmission Electron Microscopy Capabilities Brightfield,Darkfield, Difraction z-contrast Accelerating Voltage 200 kV Magnification 2,000,000 X SEI Resolution 2.4 Angstroms Measurement of Particle Size Distribution Hitachi HD 2000 Scanning Transmission Electron Microscope Specifications ( at NCSU Engineering Graduate Research Center) from NCSU Analytical Instrumentation Facility website

  13. Drug-loading efficiency determination of coQ10 by HPLC Sample preparation: -Freeze dry the particles -Extract coQ10 with methylene chloride -Dissolve further in ethanol Quantitation of coQ10: -Prepare calibration solutions of coQ10 in ethanol -Plot peak areas vs concentrations of calibration solutions -Compute for the amount of coQ10 in sample from peak area From www.waters.com Sample calibration curve

  14. Measurement of UV stability of coQ10 by HPLC Freeze dry the particles Expose the particles to UV ( 320-400 nm) Extract the coQ10 Analyze by HPLC Chromatogram of coQ10 & degradation products ( from Michael Stiff, NCSU Crop Science Department)

  15. Measurement of thermal stability by DSC Sample preparation: Freeze drying Thermal scan: Rate: 10° C per min Range: -10 to 160° C TA2010 Thermal Analyzer (TA Instruments, New Castle, DE) Compare Tg and DHfusion for coQ10, EC and coQ10-EC particles

  16. Experiment Technique Particle Size Distribution DLS STEM Drug-loading Efficiency HPLC UV Stability Thermal stability DSC Characterization methods of coQ10-loaded EC nanoparticles

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