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ETH Zürich Separation Technology. 03. Dec 2013. Chiral Column Technology New Generation of Cellulose and Amylose Columns. Smaller particle size (5µm) High loading capacity Pressure stable up to 300 bar Faster separation Save solvent Stable in NP, Polar Organic, SFC and RP conditions

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chiral column technology new generation of cellulose and amylose columns
Chiral Column TechnologyNew Generation of Cellulose and Amylose Columns
  • Smaller particle size (5µm)
  • High loading capacity
  • Pressure stable up to 300 bar
    • Faster separation
    • Save solvent
  • Stable in NP, Polar Organic, SFC and RP conditions
  • pH stable 2-9
  • Novel selectivity
chiral column technology example 1
Chiral Column TechnologyExample 1

Chiralpak AD, 5% EtOH/Heptane

Reprosil Chiral NR, 10% EtOH/Heptane

Peak A -

chiral column technology example 11
Chiral Column TechnologyExample 1

Lux 2 Cellulose, 3% EtOH/Heptane

Lux 2 Amylose, 20% EtOH/Heptane

Peak A +

chiral column technology example 2
Chiral Column TechnologyExample 2

Chiralpak AD, 5% EtOH/Heptane

Reprosil Chiral NR, 3% EtOH/Heptane

chiral column technology example 21
Chiral Column TechnologyExample 2

The devil is in the (D)ethyl

Lux2 Cellulose, 5% EtOH/Heptane

Lux 2 Amylose, 2% EtOH/Heptane

chiral detection
Chiral Detection

The CHIRALYSER is a high performance Optical Rotation Detector for monitoring Optically Active compounds in HPLC.

Innovative flow cell design in multi path technology together with an optimized measuring wavelength performs outstanding sensitivity and excellent signal to noise ratio.

The approved Faraday Compensation, superior to all other methods, enables the CHIRALYSER - MP to detect optical active molecules not only in analytical but also in preparative LC.

Operation via touch screen or remote controlled by host computer adapts the CHIRALYSER to any chromatography system.

column lifetime
Column Lifetime
  • dry solvents
    • < 0.01% water
  • clean samples
    • > 90% + no base line Peak in TLC
  • Pressure
    • < 30 bar
  • Chiralpak AD Nov/07 1700 runs
  • Chiralpak AD Nov/06 3200 runs
  • Reprosil Chiral NR Aug/08 2700 runs

Cost per column ~25`000 CHF

Cost per sample 10-20 CHF

250

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