Making a microscope slide
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1 of 8. Peter Loader @ TLT. The date today is…. Making a microscope slide. 2 of 8. Peter Loader @ TLT. Aim of the experiment.

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Making a microscope slide l.jpg

1 of 8

Peter Loader @ TLT

The date today is…

Making a microscope slide


Aim of the experiment l.jpg

2 of 8

Peter Loader @ TLT

Aim of the experiment

The aim of the experiment was to make a microscope slide of an onion skin, look at it at different magnifications and draw what we saw. We needed to identify the cell walls, nucleus and the cytoplasm.


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Peter Loader @ TLT

Safety

To be safe we wore goggles and were very careful how we used the scalpel. Because the glass could have broken we were very careful how we held it.


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Peter Loader @ TLT

Apparatus

In this experiment we used a microscope, a glass slide, a cover slip, a pair of forceps and a piece of onion skin.


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Peter Loader @ TLT

Method

We cut open an onion and took a piece of very thin skin. We placed this onto the middle of a microscope slide.

Then we put a thin piece of glass called a cover slip on top of the onion skin and gently pressed it flat.

After that we put the slide under a microscope with the lens set at low magnification.


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Peter Loader @ TLT

Method

We turned the focus dial until we could clearly see the skin. We used a pencil to draw what we saw and labelled it with the magnification.


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Peter Loader @ TLT

Results

This is what we saw.

The higher the magnification we used the bigger the cells became.

At 20 x magnification

At 50 x magnification

At 100 x magnification

Close up of onion skin

Cell wall

Cytoplasm

Nucleus


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Peter Loader @ TLT

Our conclusions

The microscope let us see the onion skin at different magnifications.

At x 20 magnification it still looked quite far away but we could see some cells.

At x 50 magnification we could see the cells clearly.

At x 100 magnification we could see individual cells, and the cellwalls containing the cytoplasm.


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