html5-img
1 / 12

Incorporating research into a laboratory course

Incorporating research into a laboratory course. 1993 - MBB changed its major to expose students to research Require all students to do an independent research project Needed to develop a course to train students in the basic techniques they would use in the lab

shanon
Download Presentation

Incorporating research into a laboratory course

An Image/Link below is provided (as is) to download presentation Download Policy: Content on the Website is provided to you AS IS for your information and personal use and may not be sold / licensed / shared on other websites without getting consent from its author. Content is provided to you AS IS for your information and personal use only. Download presentation by click this link. While downloading, if for some reason you are not able to download a presentation, the publisher may have deleted the file from their server. During download, if you can't get a presentation, the file might be deleted by the publisher.

E N D

Presentation Transcript

  1. Incorporating research into a laboratory course • 1993 - MBB changed its major to expose students to research • Require all students to do an independent research project • Needed to develop a course to train students in the basic techniques they would use in the lab • Introduction to Research (694:315, 447:315, 694:215)

  2. Course Design - Open ended research project Wanted to avoid cookbook exercises with known outcomes. Needed the element of discovery Needed to develop a project with: 1) Experiments that all students could carry out 2) Would give each student different answers

  3. Intro. to Research Project: Analysis of clones from a cDNA Library Students pick random clones and purify plasmids

  4. PvuII 719 bp PvuII SP Primer 200 bp XP Primer Students perform restriction digest and PCR analysis to determine the size of the cDNA insert PvuII digests PCR Students learn different approaches to the same question Must deal with discrepancies in the data

  5. Evaluate DNA Sequence Data Need to make subjective judgments

  6. Conduct Bioinformatic Searches of the International Scientific Databases Evaluate alignment data

  7. Determine the function of the genes

  8. Students publish their DNA sequences in the databases Student >200 sequences on NCBI

  9. Lecture/Lab format on the same day works best

  10. Students go through at least two rounds of each experiment • Gives the students a chance for success • Gives the student a chance for failure • Opportunity to work through a problem - trouble shoot • Reinforces the concepts and procedures • Students learn that science often requires repetitive • use of a technique to answer different questions.

  11. Other considerations: Need to have experiments well tested Expect problems Expensive - Money - Equipment, reagents, sequencing Time - Faculty are in the lab, safety, trouble shooting One to one interactions with the students Need to revise and update procedures/experiments Past - Radioactive sequences with high voltage gels Present - Sequencing services for individual clones Future - High throughput massively parallel sequencing

  12. Research in a lecture course Students must determine which gene on a clone is involved in a specific biological process (cell cycle, meiosis, replication etc.) Genomic DNA Given short DNA sequences at the ends Use the genomic databases to determine which genes are on the clone Examine gene expression, interaction (2-hybrid, co-IP), mutant phenotype public databases

More Related