Announcements. Grading of Mendel papers: A= completeness of answer (40); F = flow and organization (15); R = references (10); S = spelling and grammar (10).
Grading of Mendel papers: A= completeness of answer (40); F = flow and organization (15); R = references (10); S = spelling and grammar (10).
Specifics on reading assignments: Ch. 11: Skip, p. 304, btm. 309- top 312; Ch. 12: skim 327-328; skip btm 335-336; skip recombination on 338-341; Ch. 13:
1. Eukaryotic DNA replication is complex
2. The “end” problem and telomerase: aging and cancer
3. The Genetic Code - theoretical evidence for triplet code; genetic evidence using mutagens, ie. insertions and deletions can cause frameshift mutations
The Genetic Code - biochemical evidence
Prokaryotic Promoter Lies Just Upstream (5’) of Transcribed Region; RNA Polymerase Binds Two Places
-10 TATA Box
Initiation of Prokaryotic Transcription Requires Binding of Sigma Factor to Pol
Note: No primer needed
5’ to 3’
Beads with negative
charge; some proteins bind strongly, most don’t.
Add nuclei, containing
Elute with Na+ gradient
+ 1 ug/ml
0 10 20 30 40 50
Pol II, Basal TFs bind
Cis-regulatory Elements may be located thousands of bases away; Regulatory TFs bind.
Binding of Eukaryotic RNA Pol II Requires Binding of Basal TFs to Core Promoter
Pre-mRNA (primary transcript)
Poly A tail