Lecture 13: Managing the Malolactic Fermentation. Reading Assignment: Chapter 6, pages 251-261. The Malolactic Fermentation. Requires NAD + , Mn ++ Occurs after exponential growth phase Used to generate energy. Energy Generation from the Malolactic Conversion. Lactate Malate.
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Managing the Malolactic Fermentation
Chapter 6, pages 251-261
Proton Motive Force
The conversion of malate to lactate and accompanying “fixing” of a proton decreases the proton content of the cytoplasm upon efflux of lactate thereby creating a “proton motive force” across the membrane; the energy of the proton movement can then be captured in ATP.
Do you want the MLF?
Inoculated versus Spontaneous Malolactic Fermentation
Timing of Malolactic Fermentation
Choice of Strain
Method of Monitoring MLF
* Lactate can be produced from other sources
Alternative Method of Acid Reduction
To have all microbial activity finished prior to bottling.