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14_Wisium_SPE-OILIS-TR-RT-SH-RD-2020-CAN-EN-V1

Evaluation of OILIS effects on rumen fermentation, coccidiosis prevalence and zootechnical performances of lambs

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14_Wisium_SPE-OILIS-TR-RT-SH-RD-2020-CAN-EN-V1

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  1. Trial report 14_Wisium_SPE-OILIS-TR-RT-SH-RD-2020-CAN-EN-V1 Fattening lambs Evaluation of OILIS effects on rumen fermentation, coccidiosis prevalence and zootechnical performances of lambs Type of trial: R&D trial – CRF Location: Canada Date: 2020 Introduction This trial was conducted in order to evaluate the effect of OILIS on rumen fermentation, anti- oxidative status and performances of lambs in comparison to other products commonly used in Ruminants to enhance those parameters ionophores and competitors’ products. Materials and methods Animals: Total of 25 females and 25 males Suffolk x Canadian Arcott crossbreed finishing lambs. Period: Diet supplementation during 100 days Diets: 16:84 ratio forage : concentrate pelleted barley-based diet (18% protein, 19% NDF, 36 to 40% starch) Polysorbate group Plant extracts competitor Negative Control Positive Control OILIS group NC PC POLY OILIS PEC No additive / ionophores 0.018% Bovatec (ionophores) 0.25% of Tween 80 0.11% = 1.1 kg/T of OILIS SD 0.0125% Measures: • Fermentation data (stomach tubing) : Ruminal pH, Protozoa, n x105,Total VFA (mM), Acetate (A) and A:P ratio • Coccidiosis prevalence at day 1; 30, 60 and 90 i.e prevalence (%) = % of lambs with active oocyst excretion of ≥ 500 OPG • Zootechnical results : Dry Matter Intake (kg/d), Dry Matter Intake (% Body weight) and Average Daily Gain (kg/d) Date: 1st Sept. 2021 - Page: 1/4

  2. Trial report 14_Wisium_SPE-OILIS-TR-RT-SH-RD-2020-CAN-EN-V1 Results •Fermentation data: PEC 5.84 1.80 93.11 39.39 2.60 P-value 0.11 <0.01 0.01 0.03 0.02 NC 5.79 0.98 93.42 40.73 2.60 PC 5.73 0.38 97.35 35.57 2.17 POLY 5.81 1.03 98.39 40.83 2.29 OILIS 6.07 2.16 77.92 29.91 2.39 Ruminal pH Protozoa, n x105 Total VFA (mM) Acetate (A) A:P ratio The lowest acidic pH was observed for the OILIS group. ▪ A higher protozoa counts is observed for the OILIS group. ▪ This result shows OILIS has the ability to promote efficiently the flora multiplication and growth, resulting in better rumen health status and nutritional benefits which can be explained by the fact that less negative impacts on rumen flora happened, resulting in a better rumen health status ▪ A lower total VFA production (acetate) was observed for the OILIS group, linked to a lower acetate production vs the other groups. ▪ Leading, by consequence to a lower Acetate/Propionate ratio vs the Negative Control and the competitor’s product. Date: 1st Sept. 2021 - Page: 2/4

  3. Trial report 14_Wisium_SPE-OILIS-TR-RT-SH-RD-2020-CAN-EN-V1 •Coccidiosis prevalence ▪ Lambs fed OILIS diets had the lowest global prevalence of Eimeria infection (23%), while Negative Control and Positive Control presented levels of 50 and 40%, respectively. ▪ Lambs receiving Tween 80 and the competitor’s product also presented a lower prevalence of Eimeria than Negative Control. •Zootechnical results : NC 1.42 3.51 0.33 PC 1.40 3.45 0.31 POLY 1.33 3.32 0.28 OILIS 1.48 3.68 0.33 PEC 1.30 3.30 0.25 P-value <0.01 <0.01 0.06 Dry Matter Intake (kg/d) Dry Matter Intake (% BW) Average Daily Gain (kg/d) ▪ OILIS group showed the highest intake and ADG (i.e similar to NC) among the groups. ▪ No effect observed on carcass characteristics for all the groups. Date: 1st Sept. 2021 - Page: 3/4

  4. Trial report 14_Wisium_SPE-OILIS-TR-RT-SH-RD-2020-CAN-EN-V1 Conclusion This study aimed to evaluate the effect of OILIS, polysorbate (Tween 80) and a competitor’s product on rumen fermentation, coccidiosis prevalence and zootechnical performance of lambs. Ruminal pH, molar proportions of individual VFA (except for acetate) and ammonia concentration were similar among treatments, with the lest acidic one observed for the OILIS group. The total VFA concentration was lower (P < 0.01) for lambs receiving OILIS, while protozoa numbers were increased. No differences among diets were observed in coccidia oocyst counts (OPG) in feces, however, lambs of OILIS group exhibited a reduced prevalence of infection and had the highest (P < 0.01) DMI (1.48 kg/d) and the an ADG similar to the negative control group. Date: 1st Sept. 2021 - Page: 4/4

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