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APh161 - Lecture 15: Molecular Motors

Rob Phillips California Institute of Technology. APh161 - Lecture 15: Molecular Motors. Molecular Motors. Mitotic Spindle Organization. Cilia and Flagella Assembly and Dynamics. Formation of Golgi and ER Networks. Vesicle Transport. Rogues Gallery of Motor Action: Rotary Motors.

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APh161 - Lecture 15: Molecular Motors

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  1. Rob Phillips California Institute of Technology APh161 - Lecture 15: Molecular Motors

  2. Molecular Motors Mitotic Spindle Organization Cilia and Flagella Assembly and Dynamics Formation of Golgi and ER Networks Vesicle Transport

  3. Rogues Gallery of Motor Action: Rotary Motors Show Yasuda et al. movie Show Berg movie

  4. Rogues Gallery of Motor Action: Translocation

  5. Rogues Gallery of Motor Action: Translational Motor 1

  6. Rogues Gallery of Motor Action: Translational Motor 2 - Muscles See Hugh Huxley review on website

  7. Rogues Gallery of Motor Action: Translational Motor 2 - Muscles Heuser lab – Washington University

  8. Organellar Transport (Hirokawa, Science 1998)

  9. Rogues Gallery of Motor Action: Translational Motor 2

  10. Translational Motors Kinesin Speed: 850 nm/s ATPase: 44.0 1/s Myosin V Speed: 350 nm/s ATPase: 5.0 1/s Dynein Speed: -1250 nm/s ATPase: 2.0 1/s **ALL INVOLVED IN VESICLE TRANSPORT**

  11. How We Know: Gliding Motility Assays Show Vale movie

  12. Dynamics of Molecular Motors Science, Vol 300, Issue 5628, 2061-2065, 27 June 2003 - Yildiz et al. Show Gelles, Selvin movie

  13. Stepping Kinetics Spudich et al., PNAS 2000

  14. Kinesin Data (Fisher and Kolomeisky, PNAS) Fig. 3.   Kinesin velocity as a function of [ATP] under external loads, F, fixed by a force clamp. The plots, from the top down, are for F = 0, 1.05, 3.59, 4.60, and 5.63 pN, respectively. Data from Block and colleagues (9): solid curves, N = 2 fits; dashed curves, N = 2 predictions (see text)

  15. Kinesin Data continued Fig. 4.   Fits to the data of Block and colleagues (9) (and predictions) for velocity as a function of load for fixed concentrations of ATP. Note the inflection points at low [ATP] and convex profile at saturating [ATP]. (Fisher and Kolomeisky, PNAS)

  16. Kinesin Randomness Data Fig. 5.   Randomness data from Block and colleagues (9) and theoretical fits (A) as a function of external load, F, at fixed [ATP] (note that the two data points at F 5.7 pN and [ATP] = 2 mM appear separately in Block and coworkers: see figure 4 a and b of ref. 9, respectively) and (B) as a function of [ATP] at fixed loads of, from top down, F = 5.69 pN ( ), 5.35 and 4.60 pN (dashed-line predictions), 3.59 pN ( ), and 1.05 pN ( ).

  17. Motors and States

  18. What do we mean by the states?

  19. Translocation Motors

  20. Measurements on Rate of DNA Ejection Injection rate in λ (Bohm et al.) (Baldeschweiler et al.) DNA injection from T5 into vesicle

  21. Measurements on Rate of DNA Ejection

  22. Calculating the Ejection Time The mean first passage time is

  23. The Time of Ejection receptor vesicle No protein interactions

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