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Surface Reconstruction of Blood Vessels from 3D Fluorescence Microscopy Images. Arunachalam Narayanaswamy * , Badrinath Roysam*, Barbera Cutler ¤ , Chris Bjornsson § and William Shain ¶
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Arunachalam Narayanaswamy*, Badrinath Roysam*, Barbera Cutler¤, Chris Bjornsson§ and William Shain¶
Department of Electrical Engineering*, Computer Science ¤ and Biomedical Engineering§ , Rensselaer Polytechnic Institute, Troy
Center for Neural Communication Technology ¶, Wadsworth Center, Albany
"This work was supported in part by Gordon-CenSSIS, the Bernard M. Gordon Center for Subsurface Sensing and Imaging Systems, under the Engineering Research Centers Program of the National Science Foundation (Award Number EEC-9986821)."
This project aims at doing a surface reconstruction of 3D fluorescence microscopy images with hollow blood vessels. We perform a robust hypothesis test by modeling the surface to be made of small planes separating the foreground from the background voxels.
Estimation of surface of blood vessels play a very important role in determining the distance of various cells to the blood as it is the “food source”. It also helps in identifying and classifying various cell types based on their distance to the blood vessel channel and identifying various physical features of the blood vessel.
Validation is still in the development stage. Many methods including generation of phantom images, identifying the total intensity captured by the surface have been considered.
Robust 3-D Modeling of Vasculature Imagery Using SuperellipsoidsJames Alexander Tyrrell; Emmanuelle di Tomaso; Daniel Fuja; Ricky Tong; Kevin Kozak; Rakesh K. Jain; Badrinath Roysam, Medical Imaging, IEEE Transactions on, Vol.26, Iss.2, Feb. 2007, Pages:223-237
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Andrew Kuprat and Ahmed Khamayseh. Journal of Computational Physics, vol. 172, pp. 99-118 (2001).
Original 5 channel image of the hippocampus
Zoomed wireframe model of a blood vessel
Badrinath Roysam , Professor
Dept. of Electrical, Computer, and Systems Engineering
Rensselaer Polytechnic Institute, 110 8th Street, Troy, NY 12180
Phone: (518)276-8067; Fax: 518-276-8715;
Email: [email protected]
A model of alternate hypothesis
Curvature mapped model ( a small vessel)
Decimated ( Reduced ) surface