Tryptic peptides - PowerPoint PPT Presentation
Proteomics and mass spectrometry
Proteomics and mass spectrometry. Manimalha Balasubramani. Outline. Mass spectrometers Protein identification Quantitative proteomics Protein-protein interactions. 1258.5603. 100. 1303.7007. 6.3E+4. 90. 80. 70. 60. 878.4913. % Intensity. 1031.5374. 983.4860. 50.
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32 P radioactivity ( cpm )
5000. 80. 50. 32 P radioactivity ( cpm ). Acetonotrile (% ). 2000. 30. 0. 0. 8000. 80. 5000. 50. 32 P radioactivity ( cpm ). Acetonotrile (% ). 30. 2500. 0. 14000. 80. 10000. 50. 32 P radioactivity ( cpm ). Acetonotrile (% ). 30. 5000. 0. 0. 10. 20. 30. 40.
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Supplemental Fig. S1
Supplemental Fig. S1. a). b). PsAPY1 (AB071369) 1:MEFLIKLIT-FLLFSMPAITSSQYLGNNLLTSRKIFLKQEEISSYAVVFDAGSTGSRIHV 59 MtAPY1;1 (AY180377) 1:MEFLITLITTVLLLLMPAITSSQYLGNNLLTNRKIFQKQETISSYAVVFDAGSTGSRIHV 60 ***** *** ** **************** **** *** *******************
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Tryptic digestion
Centre for Protein Research, Department of Biochemistry, University of Otago Torsten Kleffmann (Research Fellow) , Joanne Preston (Technician) , Diana Carne (Technician). Proteomics Workflow for Gel-based and LC-coupled Mass Spectrometry.
Table 1. Occurrence of N-X-S/T motives in tryptic peptides 1
Table 1. Occurrence of N-X-S/T motives in tryptic peptides 1. 1. This calculation is based on the Swiss-Prot database (ver. 04.11). 2. Proteins containing N-X-S or N-X-T sequons in their amino acid sequences. 3. Proteins annotated as “glycoprotein” in the DB entries.
PEPTIDES
PEPTIDES. Tissue damage, allergic reactions, viral infections and other inflammatory events activate a series of proteolytic rections that generate BK and KD in the circulation or tissues
Peptides
Peptides. Martina Zlámalová. Peptide Synthesis Methodology. is carried out by FMOC chemistry, using PEG-Polystyrene resins peptides are cleaved from the resin and de-protected using cleavage cocktail peptides are precipitated from the cocktail using cold diethyl ether
Peptides
References listed in Table 1S Akoun, G.M. et al. (1985. Serum neurone-specific enolase, A marker for disease extent and response to therapy for small-cell lung cancer. Chest. 87, 39-43
Peptides
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PEPTIDES
PEPTIDES. Tissue damage, allergic reactions, viral infections and other inflammatory events activate a series of proteolytic rections that generate BK and KD in the circulation or tissues
Ghrelin Peptides
Creative Peptides is one of the primary suppliers of diabetes related peptides with a range of more than 100 peptides, from milligrams to kilograms, generic standard to cGMP (current Good Manufacturing Practice) standard.https://www.creative-peptides.com/research-areas/peptides-in-diabetes.html\n\n
Antimicrobial Peptides
Antimicrobial Peptides. An ancient immune system. Antimicrobial peptides: What are they?. Small proteins of cationic charge involved in host innate immune defense. Antimicrobial peptides: Where are they?. In everything from Amoebas to Humans (incl. Bacteria), Abundant in vertebrates in:
Collagen peptides
With age, collagen an abundant protein in your body reduces and your skin loses elasticity. To regain the health of your skin you need collagen peptides or supplements to balance the amount of collagen required by the body. Collagen Hydrolysate Powder is one such supplement that can benefit you immensely. Include collagen in your daily nutrition and experience great benefits. For more information on quality collagen supplement visit our site now!