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QUESTIONS: 1. Name the DNA for which transcription requires SP1.

METHOD 1. Set up in vitro transcription reaction with DNA template, 32P-UTP, plus or minus transcription factor SP1 2. Separate transcripts by gel electrophoresis. QUESTIONS: 1. Name the DNA for which transcription requires SP1. 2. What is the evidence as seen in the autoradiogram?

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