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Immunolabeling of Cells and Transmission Electron Microscopy

Immunolabeling of Cells and Transmission Electron Microscopy

Immunolabeling of Cells and Transmission Electron Microscopy. Ike Miguel June 30, 2006 ABE Workshop 2006. Purpose. Using TEM to label specific sites in Arabidopsis thaliana. Goal Overview of Immunolabeling Preparation of samples and viewing Observations of results Thoughts.

By seanna
(593 views)

Jui-Ming (Ryan) Yang

Jui-Ming (Ryan) Yang

Label-Free Protein Biosensor Based on Aptamer-Modified Carbon Nanotube Field-Effect Transistors Kenzo Maehashi, Taiji Katsura, Kagan Kerman, Yuzuru Takamura, Kazuhiko Matsumoto, and Eiichi Tamiya Anal. Chem. 2007, 79, 782-787. Jui-Ming (Ryan) Yang. Outline. Background CNT applications

By khuyen
(210 views)

Transcription

Transcription

Transcription. RNA Polymerases and General Transcription Factors. Subjects, covered in this lecture. A short overview Structure and mechanism of RNA polymerases Eukaryotic promoters General transcription factors Elongation factors

By libitha
(283 views)

Ch 9:T cell receptor

Ch 9:T cell receptor

Ch 9:T cell receptor. Where we’re going. Structure of the TCR- alpha and beta chains ( αβ ) The γδ Tcells- know a bit about them The CD3 components and signal transduction-cover much, responsible for just a few concepts. Alloreactivity. Review of TCR’s.

By cicely
(149 views)

1 brood

1 brood

total CORT. 60. 50. 40. Total CORT (ng/ml). 30. 20. 1 brood. 10. 0. 0-3 minutes. 30 minutes. 600. CBG capacity. 500. 400. Specific Binding (nM). 300. 200. 100. 2-3 broods. 0. pugetensis. oriantha. gambelii. 3.5. Free CORT. 3. 2.5. 2. Free CORT (ng/ml). 1.5. 1.

By ciel
(228 views)

Chemical syntheses of nu c leob ases , nu c leosid es , nu c leotid es a oligonu c leotid es

Chemical syntheses of nu c leob ases , nu c leosid es , nu c leotid es a oligonu c leotid es

Chemical syntheses of nu c leob ases , nu c leosid es , nu c leotid es a oligonu c leotid es. Synthesis of pyrimidine bases. Transforma tions of pyrimidine bases. Syntheses of puri ne bases. Transforma tions of puri ne bases. Synthesis of nu c leosid es. Synthesis of nu c leosid es.

By freira
(67 views)

Rapid Stain-Free Western Blotting with the V3Western Workflow™

Rapid Stain-Free Western Blotting with the V3Western Workflow™

Rapid Stain-Free Western Blotting with the V3Western Workflow™. Biotechnology Explorer TM Program. Rapid V3 Stain-Free Western Blotting Workshop Outline. Introduction to Rapid Blotting and V3 Stain-Free Protein Gel Electrophoresis Stain-Free Gel Activation Western Transfer

By ervin
(593 views)

Immunophenotyping and applications of cell analysis in the hematology laboratory

Immunophenotyping and applications of cell analysis in the hematology laboratory

Immunophenotyping and applications of cell analysis in the hematology laboratory. J.Paul Robinson Professor of Immunopharmacology & Biomedical Engineering April 5, 2005. What can flow cytometry be used for?. Clinical and Research. Immunology Hematology Pathology Microbiology Genetics.

By alaura
(311 views)

Functional Neuroimaging Lab School of Psychology University of Newcastle

Functional Neuroimaging Lab School of Psychology University of Newcastle

Comparison of methodologies for the assessment of dopamine receptor binding in subregions of the striatum. Sharna Jamadar Mentor : Julie Price PET Modality Coordinator: Jonathan Carney. Functional Neuroimaging Lab School of Psychology University of Newcastle

By ulric
(133 views)

MCB- Signal Transduction Lecture 1

MCB- Signal Transduction Lecture 1

MCB- Signal Transduction Lecture 1. General Concepts of Signal Transduction Cell Communication Types of Receptors Molecular Signaling Receptor Binding Scatchard Analysis Competitive Binding Second Messengers. Signaling throughout Evolution. Bacteria Sense nutrients

By otis
(187 views)

Molecular interactions observed in a real time

Molecular interactions observed in a real time

Molecular interactions observed in a real time. – possibilities and limitations of SPR technology. Bogdan Walkowiak Department of Biophysics Technical University of Lodz and Department of Molecular and Medical Biophysics, Medical University of Lodz. Endpoint assay.

By menora
(113 views)

plasma

plasma

K 1. k 7. K 5. k 3. k 2. k 8. k 6. k 4. Full 6-compartments. injection. t=0. V. IV. plasma. II. III. K 9. urine. K 1. K 2. Extracellular Fluid (ECF). injection. t=0. plasma. ECF. K 9. urine. K 1. K 5. k 2. k 6. Slow Space. injection. t=0. plasma. ECF. slow. K 9.

By armen
(105 views)

Kd or IC50: concentration that gives 50% maximal binding; measure

Kd or IC50: concentration that gives 50% maximal binding; measure

TYPICAL BINDING CURVE. Maximum Number of receptors. SPECIFIC BINDING (NUMBER OF RECEPTORS OCCUPIED). Kd. Concentration of Drug Used. Kd or IC50: concentration that gives 50% maximal binding; measure of AFFINITY of the drug for the receptor. efficacy. ED50.

By ezra-daniel
(346 views)

O Duss et al. Nature 000 , 1-5 (2014) doi:10.1038/nature13271

O Duss et al. Nature 000 , 1-5 (2014) doi:10.1038/nature13271

Sequential and specific binding of the RsmE protein to the RsmZ sRNA. O Duss et al. Nature 000 , 1-5 (2014) doi:10.1038/nature13271.

By anneliese-panagos
(75 views)

magnetic beads kit

magnetic beads kit

http://www.cd-bioparticles.com/l/Magnetic-Particles-Conjugation-Kits_20_307_0.html\n

By thomasm2014
(61 views)

Magnetic particles conjugation kits

Magnetic particles conjugation kits

With high efficacy and non-specific binding ability, our fast and easy magnetic particles conjugation kits exhibit promising properties as useful tools for antibodies and other biomolecules conjugation processes. https://www.cd-bioparticles.com/product/magnetic-particles-conjugation-kits-list-21.html\n

By bioparticlesmelly
(0 views)

Chromatography

Chromatography

Chromatography. Desalting and Affinity. Chromatography. Technique to separate components of a mixture by passing them through a matrix. A solvent is used for carrying sample mixture through the matrix Separation occurs because each compound in a mixture interacts differently with the matrix.

By matthewperry
(2 views)

concepts for discussion synopses – don’t overdo it

concepts for discussion synopses – don’t overdo it

concepts for discussion synopses – don’t overdo it how comfortable were you with Wagner paper, Mintun , Farde ? non-specific vs specific binding how to measure non-specific binding (displacement) can we do displacement in humans? maybe… qualitative imaging vs quantitative imaging

By jmccormack
(0 views)

Lecture 21 Vesicular Traffic

Lecture 21 Vesicular Traffic

Lecture 21 Vesicular Traffic. reading: Chapter 14. Signaling at the Surface reading: Chapter 15. ER vesicle budding and fusion to form new cisterna retrograde return of ER-resident proteins cysternal progression and maturation secretion – constitutive, regulated

By lanel
(1 views)


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