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Culture and identification of infectious agents, Lecture 33

Culture and identification of infectious agents, Lecture 33

Culture and identification of infectious agents, Lecture 33. Dr. Alvin Fox. Key Terms. After culture Biochemical (physiological) tests Genetic tests Sequencing, Polymerase chain reaction (PCR) DNA-DNA homology Restriction enzymes (digests) Chemical - fatty acid/protein profiling

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412 views • 30 slides
Rapid and sensitive detection of MRSA  by PCR-based methods in the environment and hospital patients.

Rapid and sensitive detection of MRSA  by PCR-based methods in the environment and hospital patients.

Rapid and sensitive detection of MRSA  by PCR-based methods in the environment and hospital patients. by Dr. Corinne Whitby Department of Biological Sciences, Wivenhoe Park, University of Essex, Colchester, Essex, CO4 3SQ. Tel: +44 (0) 1206 872062, Fax: +44 (0) 1206 872592.

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458 views • 17 slides
University of Washington San Francisco Department of Health St. James Hospital, Dublin, Ireland

University of Washington San Francisco Department of Health St. James Hospital, Dublin, Ireland

Azithromycin resistance in Treponema pallidum in the United States and Ireland is associated with a mutation in the 23S rRNA gene. University of Washington San Francisco Department of Health St. James Hospital, Dublin, Ireland Johns Hopkins University. Major points.

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265 views • 17 slides
Lab 8

Lab 8

Lab 8. Restriction Digestions. Biotechnology. Using a restriction enzyme to cut DNA. Restriction Enzymes. Sticky ends: EcoRI 5’-GAATTC-3’. G + AATTC. 3’-CTTAAG-5’. CTTAA-5’ G. PstI 5'-CTGCAG-3' CTGCA-3’ + G.

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235 views • 10 slides
Lab 8

Lab 8

Lab 8. Restriction Digestions. Biotechnology. Using a restriction enzyme to cut DNA. Restriction Enzymes. Sticky ends: EcoRI 5’-GAATTC-3’. G + AATTC. 3’-CTTAAG-5’. CTTAA-5’ G. PstI 5'-CTGCAG-3' CTGCA-3’ + G.

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254 views • 10 slides
Cloning a DNA segment from bacteriophage

Cloning a DNA segment from bacteriophage

Cloning a DNA segment from bacteriophage. Recombinant DNA transformed into bacterial cells Last week we plated cells onto agar plates + ampicillin + X-gal Controls: E.coli-pUC18 “negative control” Should only get blue colonies E.coli-pUC18-bacteriophage “positive control”

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273 views • 7 slides
Abstract

Abstract

Regulation of Caspase-3 by PKC δ. Sean Crisafi 1 , Yadira Malavez 4 , and Andrea Doseff 2,3

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149 views • 1 slides

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