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Ph step gradient - PowerPoint PPT Presentation

Protein Identification using 2D LC/MS/MS based on pH Gradient and Reverse Phase Separation

Protein Identification using 2D LC/MS/MS based on pH Gradient and Reverse Phase Separation. Column Technology Inc.,. Part I. Comparison of salt and pH step gradient. pH Based 2D-LC-MS/MS: pH 3.0-8.0, Buffer (5-10mM) v.s. Salt Based 2D-LC-MS/MS: salt 0-2000 mM, pH ~2.5.

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277 views • 13 slides

Phosphopeptides identification

Phosphopeptides identification. Column technology Inc., WWW.columntechnology .com. SAX for phosphopeptide separation. Phosphopeptides have lower pIs then non-phosphopeptides. Off line pH gradient follow by reverse phase separation. Peptides fractionated by pH step gradient

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154 views • 8 slides

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Ph step gradient - PowerPoint PPT Presentation

Protein Identification using 2D LC/MS/MS based on pH Gradient and Reverse Phase Separation

Phosphopeptides identification