'Peptide identification' presentation slideshows

Protein Identification by Sequence Database Search

Protein Identification by Sequence Database Search. Nathan Edwards Department of Biochemistry and Mol. & Cell. Biology Georgetown University Medical Center. Peptide Mass Fingerprint. Cut out 2D-Gel Spot. Peptide Mass Fingerprint. Trypsin Digest. Peptide Mass Fingerprint. MS.

843 views • 71 slides

Statistical Significance for Peptide Identification by Tandem Mass Spectrometry

Statistical Significance for Peptide Identification by Tandem Mass Spectrometry. Nathan Edwards Center for Bioinformatics and Computational Biology University of Maryland, College Park. Mass Spectrometry for Proteomics. Measure mass of many (bio)molecules simultaneously High bandwidth

1.04k views • 64 slides

HighGlycan Meeting Split 2013

725 views • 60 slides

Proteomic Characterization of Alternative Splicing and Coding Polymorphism

Proteomic Characterization of Alternative Splicing and Coding Polymorphism. Nathan Edwards Center for Bioinformatics and Computational Biology University of Maryland, College Park. Mass Spectrometry for Proteomics. Measure mass of many (bio)molecules simultaneously High bandwidth

592 views • 51 slides

Improving the Sensitivity of Peptide Identification for Genome Annotation

Improving the Sensitivity of Peptide Identification for Genome Annotation. Nathan Edwards Department of Biochemistry and Molecular & Cellular Biology Georgetown University Medical Center. Why Tandem Mass Spectrometry?.

579 views • 49 slides

Improving Genome Annotation using Proteomics

Improving Genome Annotation using Proteomics. Nathan Edwards Center for Bioinformatics and Computational Biology University of Maryland, College Park. Mass Spectrometry for Proteomics. Measure mass of many (bio)molecules simultaneously High bandwidth

562 views • 39 slides

Improved proteomic analysis pipeline for LC-ETD-MS/MS

Improved proteomic analysis pipeline for LC-ETD-MS/MS. Xie Li qi. F ragmental pattern of Protein backbone in MS. b, y products are formed by the lowest energy backbone cleavage of protein ions. c, z cleavage occurs between almost any combination of amino acids, except for cyclic N of Pro.

528 views • 36 slides

Improving the Sensitivity of Peptide Identification

419 views • 31 slides

Mass Spectrometry at The University of Louisville HSC

355 views • 20 slides

Improving Statistical Significance Assignment in Mass Spectrometry Based Peptide Identification

Improving Statistical Significance Assignment in Mass Spectrometry Based Peptide Identification. Overview Statistical Significance in Peptide Identification Statistics through deNovo method Example New way to Combine Search Results. Yi-Kuo Yu

330 views • 18 slides

Scaffold

476 views • 18 slides

Glycomics project overview

Glycomics project overview. Life Science Ontologies. Glyco An ontology for structure and function of Glycopeptides 573 classes, 113 relationships Published through the National Center for Biomedical Ontology (NCBO). ProPreO

235 views • 15 slides

Generalized Protein Parsimony

Generalized Protein Parsimony. Nathan Edwards Department of Biochemistry and Molecular & Cellular Biology Georgetown University Medical Center. Traditional Protein Parsimony. Select the smallest set of proteins that cover all identified peptides . Sensible principle, implies

131 views • 6 slides

Probe4

A. MS/MS of 1032.17, 2+. Probe4. #. 3759. RT:. 35.77. NL:. 3.98E4. F:. ITMS + c NSI d Full ms2 1032.17@30.00 [ 270.00-2000.00]. 100. 983.1. 95. 90. 85. 80. FQSpEEQQQTEDELQDK Xcorr: 5.20. 75. 70. 65. 60. 55. 50. Relative Abundance. 45. 40. y 13 +2 810.5.

134 views • 3 slides

For five columns, line up guides with these boxes

Georgetown University. Peptide Atlas A8_IP LTQ MS/MS Dataset Tryptic search of Human ESTs using PepSeqDB [2] 107084 spectra searched ~ 26 times: - Target + 2 decoys, 5 engines, 1+ vs 2+/3+ charge. 8685 search jobs, 25.7 days of total CPU time. 5211 TeraGrid TKO jobs in < 2 hours (143 nodes)

81 views • 1 slides

Spectral matching identifies peptides by comparing spectra with libraries of identified spectra

D1. D2. D3. D4. I0. I1. I2. I4. I3. Begin. b1. y1. b2. y2. End. HMMatch : Peptide Identification by Spectral Matching of Tandem Mass Spectra Using Hidden Markov Models. US HUPO.

168 views • 1 slides

For five columns, line up guides with these boxes

Georgetown University. Improving the Sensitivity of Peptide Identification from Tandem Mass Spectra using Meta-Search, Grid-Computing, and Machine-Learning. Nathan J. Edwards, Georgetown University Medical Center. Annual Meeting, 2009. Introduction.

110 views • 1 slides

Improving the Sensitivity of Peptide Identification With Meta-Search and Machine Learning

Georgetown University. D 1. D 2. D 3. D 4. I 0. I 1. I 2. I 4. I 3. Begin. b 1. y 1. b 2. y 2. End. Improving the Sensitivity of Peptide Identification With Meta-Search and Machine Learning. Nathan J. Edwards 1 , Xue Wu 2 , Chau-Wen Tseng 2.

169 views • 1 slides

HMM-based spectral matching can be used to identify peptides.

ion peak non-ion peak. D1. D2. D3. D4. I0. I1. I2. I4. I3. Begin. b1. y1. b2. y2. End. Peptide Identification by Spectral Matching of Tandem Mass Spectra Using Hidden Markov Models. RECOMB Satellite Conf.

135 views • 1 slides

Introduction

Georgetown University. OMICS 17 Protein Mix LCQ MS/MS Dataset Semi-tryptic search of SwissProt 39408 spectra searched ~ 36 times: - Target + 2 decoys, 7 engines, 1+ vs 2+/3+ charge. 3969 search jobs, weeks of CPU time. Total elapsed time (Mascot bottleneck): < 28 hours.

63 views • 1 slides