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Finding the protein interacting with his-tagged Orf1 in M.smegmatis

Finding the protein interacting with his-tagged Orf1 in M.smegmatis. Orf1 Constructing Yeast two hybrid vector of EF-tu Preparing of Nothern blotting (confirming the expression of EF-Tu in 7H9+0.2%glucose or 7H9 30% CO ). Orf2 Transfomation pMsm orf2 vector in BL21

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220 views • 13 slides

Finding the protein interacting with his-tagged Orf1 in M.smegmatis

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210 views • 12 slides

cutB

? Southern blotting (probe : ORF2L PCR product). probe(970 bp). orf6. orf2. orf5. orf4. cutB. cutC. cutA. Pst I (2290 bp). Nco I (5223 bp). Nco I ? Cla I (4701 bp). probe(970 bp). orf6. orf2. orf5. orf4. cutB. cutC. cutA. Pst I (2290 bp). Nco I (5223 bp). Cla I (5121 bp).

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275 views • 10 slides

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Orf2 - PowerPoint PPT Presentation

Finding the protein interacting with his-tagged Orf1 in M.smegmatis

Finding the protein interacting with his-tagged Orf1 in M.smegmatis

cutB