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Genome-wide screen for CRAC channels

Genome-wide screen for CRAC channels. Genome-wide RNAi screen for SOC influx. Treat 10 4 S2 cells 5 days with dsRNA; load with fluo-4, wash 2 Ca 2+ Ringer; 3 fluorescence measurements per well: basal , then add 1 m M TG; CCE , then lyse cells; F max

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Genome-wide screen for CRAC channels