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Cell death. Part II: Regulation. Eric R. Gauthier, Ph.D. Dept. Chemistry-Biochemistry. Outline. Basic regulatory mechanisms: Turnover; Compartmentalization; Alternative splicing; Changes in protein conformation; Post-translational modifications; Protein-protein interactions.
Normal cells that are subject to a damaging stimulus may become sublethally damaged. If the stimulus abates, cells recover but if it continues, cells die and undergo necrosis. Massively damaging stimuli, e.g. great heat or strong acids, cause immediate death of
Cell Death. Hoon Lee. Cell Death. Within each cell line, the control of cell number is regulated by a balance of cell proliferation and cell death. There are two principle patterns of cell death 1. Necrosis (death by injury) 2. Apoptosis (death by suicide). Necrosis.
+. +. GluR1/2. GAPDH siRNA. -. +. A. B. Neuron. (Percentage of Total). Cell Death. (Percentage of Total). Cell Death. 100. 80. *. 60. Cell Death. (Percentage of Total). 40. C. 20. 0. HEK 293T Cells. +. +. GluR1/2. E. -. +. Anti-GAPDH. GAPDH siRNA. Propidium iodide
CELL DEATH . INJURY. SUICIDE. APOPTOSIS : event series. "find-me" signal". release (at least in mammalian cells) ATP and UTP. These nucleotides bind to receptors on wandering phagocytic cells like macrophages and dendritic cells and attract them to the dying cells. "eat me" signal .
Cell death?. Trypan blue stain. ALP positive cell. ALP IN SERUM. ALP IN SERUM. What else bone markers in serum?. Ostoecalcin (intact)----osteoblast function or number C-terminal telopeptides of type I collagen ----bone resorption, ostoeclast and osteoblast.
Cell Birth, Cell Death. An overview of Chapter 22 By Patty Eneff. Cell Birth. Cell division implies production of identical daughter cell that do not differ form the parent cells. If this were always the case, hundreds of differentiated cells would never be formed. Differences Occur.
CELL ADAPTATIONS CELL INJURY CELL DEATH. OBJECTIVES. Understand the 3 main anatomic concepts of disease--- Degenerative, Inflammatory, Neoplastic Understand the concepts of cellular growth adaptations--- Hyperplasia, Hypertrophy, Atrophy, Metaplasia
Implementation of a Hypothermia Treatment Program for Hypoxic Ischemic Encephalopathy- SCVMC NICU Experience.