'Lb amp' presentation slideshows

GENETIC ENGINEERING

GENETIC ENGINEERING. BIOLOGY 2/2A MOTZKO. Flavr Savr (1994). Combined tomato antisense (mutated) DNA to prevent the ripening process. GENETIC ENGINEERING. Recombinant DNA = utilizing DNA from two different sources Transgenic DNA = utilizing DNA from two different species

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Biotechnology and Genetic Engineering

Biotechnology and Genetic Engineering. Biotechnology. The use of living cells to make products such as pharmaceuticals, foods, and beverages The use of organisms such as bacteria to protect the environment The use of DNA science for the production of products, diagnostics, and research.

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pGLO Transformation LAB AP LAB 6

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pGLO Transformation LAB AP LAB 6

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Announcements

Announcements. New Weekly Schedule Observer on March 6 and 13. Measuring Zone of Inhibition. Bacterial Transformation. Student Training By Quanina Quan and UCSD ScienceBridge. Objective.

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Biotechnology

Biotechnology. Explorer Program. Serious About Science Education. Aequorea victoria. DNA. RNA. Protein. Trait. Central Framework of Molecular Biology. Transformation is a natural process that Bacterial have evolved in order to obtain DNA from their environment.

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IS THIS COOL?

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Lab 5a Transformation of Escherichia coli with pARA -R

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pGLO Bacterial Transformation, Purification and SDS gel

pGLO Bacterial Transformation, Purification and SDS gel. Timeline Tranformation. Monday1/7 Lecture and discussion Tuesday 1/8 Transformation Laboratory Transform cells and spread plates Wednesday 1/9 (FIRE) Data Collection and Analysis Observe transformants and controls

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pARA-R Sequence

Biotechnology Lab Program. Bruce Wallace. pARA-R Sequence. RFP Expression Sequence. Laboratory Protocols by:. Marty Ikkanda. Powerpoint by:. Anthony Daulo Kristi Schramm. Pierce College, Woodland Hills, CA. V.1.2a.3. pARA-R construct. Bruce Wallace. Recombinant plasmid of interest.

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pARA-R Sequence

Biotechnology Lab Program. Bruce Wallace. pARA-R Sequence. RFP Expression Sequence. Laboratory Protocols by:. Marty Ikkanda. Powerpoint by:. Anthony Daulo Kristi Schramm. Pierce College, Woodland Hills, CA. V.1.2a.3. pARA-R construct. Bruce Wallace. Recombinant plasmid of interest.

298 views • 19 slides

Genetic Engineering

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Transformation Lab

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Transforming E. coli with a Recombinant Plasmid

Transforming E. coli with a Recombinant Plasmid. What is biotechnology?. Employs use of living organisms in technology and medicine Modifying living organisms for our use Mass production Recombinant DNA Recombine DNA fragments from various sources. Introduction. Restriction enzymes

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Recombinant DNA Bacterial Transformation Student Instructions Transformation

Recombinant DNA Bacterial Transformation Student Instructions Transformation. Recombinant Transformation. Finger flick (vortex) tube “CC” to resuspend cells. Recombinant Transformation.

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Biology 11H

Biology 11H. pGLO Prep: Gene Transformation in Bacteria. Objectives. By the end of this lab you should be able to: Describe the function of plasmids in bacteria Relate genes to protein synthesis. Key Terms!. Plasmid Transformation Gene Protein DNA Agar. Lab Skills & Processes :.

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Transformation Lab

Transformation Lab. Engineering a Bacteria to Glow Green. Goal:. To get E. coli to take in a plasmid that contains the GFP gene and produce green fluorescence protein when given the right environmental signal. Plasmid. Contains : Origin of Replication Antibiotic Resistance

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pGLO plasmid

pGLO plasmid. Beta Lactamase Ampicillin resistance Green Fluorescent Protein (GFP) Aequorea victoria jellyfish gene araC regulator protein Regulates GFP transcription. ara Operon. lac Operon. araC. B. A. D. LacI. Z. Y. A. Effector (Arabinose). Effector (Lactose). araC. B. A.

2.1k views • 7 slides