'Hammerhead ribozyme' presentation slideshows

Hammerhead ribozyme - PowerPoint PPT Presentation

Part I: Ribozymes

Part I: Ribozymes

Prof. F.Allain 2/2/2004. Part I: Ribozymes. Part II: SELEX (RNA in-vitro evolution). Part III: RNAi RNA inhibition and silencing. The RNA world hypothesis. Part I: Ribozymes. A brief History. How many ribozyme ? Why ?. Catalytic efficiency, condition. 3D structure of ribozyme:

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1.06k views • 69 slides
12/1/03 11:01 PM

12/1/03 11:01 PM

12/1/03 11:01 PM. SELEX Have a random 40-mer synthesized, between 2 arbitrary 20-mers (PCR sites) 4 40 = 10 24 Practical limit = 10 15 = ~ 2 nmoles = ~ 50 ug DNA 10 15 is a large number. Very large (e.g., 500,000 times as many as all the unique 40-mers in the human genome.

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533 views • 44 slides
12/1/03 11:01 PM

12/1/03 11:01 PM

12/1/03 11:01 PM. SELEX Have a random 40-mer synthesized, between 2 arbitrary 20-mers (PCR sites) 4 40 = 10 24 Practical limit = 10 15 = ~ 2 nmoles = ~ 50 ug DNA 10 15 is a large number. Very large (e.g., 500,000 times as many as all the unique 40-mers in the human genome.

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497 views • 44 slides
The Hammerhead Ribozyme

The Hammerhead Ribozyme

The Hammerhead Ribozyme. Peter J. Mikulecky. Sid Altman. Tom Cech. What is a Ribozyme?. 1) Enzyme. 2) Ribonucleic Acid. NOT PROTEIN. 1989 Nobel Prize In Chemistry. Secondary:. Tertiary:. Structure . As with proteins, we consider. Primary:. GGCCGAACUGGUA.

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742 views • 17 slides
The Hammerhead Ribozyme

The Hammerhead Ribozyme

The Hammerhead Ribozyme. Peter J. Mikulecky. Sid Altman. Tom Cech. What is a Ribozyme?. 1) Enzyme. 2) Ribonucleic Acid. NOT PROTEIN. 1989 Nobel Prize In Chemistry. Secondary:. Tertiary:. Structure. As with proteins, we consider. Primary:. GGCCGAACUGGUA.

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223 views • 17 slides
Two Approaches of RNA Design: Fluorescent Riboswitch & Ligase Selection

Two Approaches of RNA Design: Fluorescent Riboswitch & Ligase Selection

The Schlick Group Retreat February 2008. Two Approaches of RNA Design: Fluorescent Riboswitch & Ligase Selection. Namhee Kim and Giulio Quarta Laboratory of Prof. Tamar Schlick. 1. Design and Applications of Novel RNAs 2. Novel Candidates of Fluorescent Riboswitch

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296 views • 12 slides

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