'Donor emission' presentation slideshows

Letting someone else doing my job

Letting someone else doing my job. http://probes.invitrogen.com/resources/education/tutorials/1Intro/player.html. Epi-illumination is form of Kohler Illumination: Objective is also condenser. White light (regular Kohler) Brightfield, phase, etc. Light is focused At back aperture

705 views • 59 slides

Individual Projects due today. Outline of rest of course April 9: FRET April 11: Diffusion

481 views • 34 slides

Individual Projects due today. Outline of rest of course April 9: FRET April 11: Diffusion

350 views • 34 slides

Announcements

312 views • 31 slides

Next Monday: Diffusion This afternoon: guidance on your papers.

381 views • 27 slides

213 views • 21 slides

FRET

932 views • 19 slides

Methods: Fluorescence

Methods: Fluorescence. Biochemistry 4000 Dr. Ute Kothe. Remember: Absorbance. Absorbance of monochromatic light reduces the intensity (I) Measured relatively to original intensity (I 0 )

580 views • 16 slides

HW returned today. 2. Another HW assigned yesterday, because I messed up. Due Friday.

317 views • 16 slides

FRET

FRET. Some limitation and disadvantages:. It should be conducted only within Forster distance Maximal overlap of donor emission and acceptor excitation High quantum yield of the donor Minimal direct excitation of the acceptor at the excitation maximum of the donor

404 views • 14 slides