'Caspase 9' presentation slideshows

Apoptosis

PRESENTED BY DOCTOR DEATH. Apoptosis. Learning Objectives To Die For. To understand the proteins involved in apoptosis. Know the regulation of apoptotic signaling pathways. Importance of apoptosis in the immune system and diseases. Apoptosis (Programmed cell death).

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6th International Symposium on Translational Research in Oncology

Jointly sponsored by Postgraduate Institute for Medicine and Clinical Care Options, LLC. 6th International Symposium on Translational Research in Oncology. October 11-14, 2007 Dublin, Ireland. This program is supported by educational grants from.

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Imad Fadl-Elmula

APOPTOSIS PROGRAMMED CELL DEATH. Imad Fadl-Elmula. History. Described first by Kerr et al., (1972). Apoptosis derived from Greek "falling off" (as for autumn leaves). Importance. Essential for physiological and developmental process Embryonic development.

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Dysfunction Apoptosis and Disease

?????????. Dysfunction Apoptosis and Disease. ????. 1 ??? 2 ??????????? 3 ???????? 4 ??????????. (General introduction). 1. ??. 1.1 ?? ???? ( apoptosis,Apo ): ????????????? ??????? (programmed cell death ,PCD). 1.2. Apo. ???. 1. 2.1. Apo. ??????.

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Berkeley Feb 2004

Berkeley Feb 2004. Outer membrane. Inter-membrane space. Inner membrane. Matrix. Cristae. ATP hydrolysis. ADP + Pi. H +. H +. ATP. ++. --. The proton circuit: Continuous generation of ATP. ADP + Pi. H +. H +. ATP. Energy (redox energy, photons etc). Cytochrome c at +250mV.

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Berkeley Feb 2004

Berkeley Feb 2004. Outer membrane. Inter-membrane space. Inner membrane. Matrix. Cristae. ATP hydrolysis. ADP + Pi. H +. H +. ATP. ++. --. The proton circuit: Continuous generation of ATP. ADP + Pi. H +. H +. ATP. Energy (redox energy, photons etc). Cytochrome c at +250mV.

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Chapter 17: Cell Death

Chapter 17: Cell Death. Know the terminology : Apoptosis, necrosis, Bcl-2, caspase, procaspase, caspase-activated DNAse (or CAD), death domain, cytochrome c, mitochondrial permeability transition pore, Apaf, FAS, TNF a ,. The many forms of cell death. Necrosis:

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CYTOCHROMES

BIOCHEMISTRY

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Heat Shock Proteins: Psychotherapy for the Cell

Heat Shock Proteins: Psychotherapy for the Cell. Kelly Andringa November 8, 2000. Historically:. Heat shock proteins were first discovered when cells were heated and proteins of certain molecular weight were produced. These proteins appeared with the development of cell thermotolerance.

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APOPTOSIS

APOPTOSIS. Dr Sarah Meachem. Intentions of this talk. Define apoptosis terminology, methods to detect Define necrosis terminology Understand the differences between apoptosis & necrosis. Importance of Apoptosis. Programmed cell death Regulate cell numbers eg spermatogenesis

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Bcl-2

Bcl-2. Sarah A. Norris. Apoptosis. Programmed cell death. http://virtuallaboratory.net/Biofundamentals/lectureNotes/Topic5-4_CellDeath.htm. http://upload.wikimedia.org/wikipedia/commons/8/8a/Bcl-2_Family.jpg. Bcl-2 Structure. Encodes an integral membrane protein.

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Terminal deoxynucleotidyl transferase dUTP nick end labeling

Terminal deoxynucleotidyl transferase dUTP nick end labeling. Dependency on Synaptic T arget.

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Enhancement of stress-induced apoptosis in B-lineage cells by caspase-9 inhibitor

Enhancement of stress-induced apoptosis in B-lineage cells by caspase-9 inhibitor. Nisha Shah et al., BLOOD.2004;104:2873-2878. ???? ??? 91390063. Introduction. B-lineage (BLIN) Acute lymphoblastic leukemia (ALL) cell line : BLIN-3, BLIN-4L Caspase-9 inhibitor (C9i)

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In Vitro Effect of Thai herbal extracts with anti-psoriatic activity on the expression of caspase 9

Psoriasis, known as the incurable disease, is a chronic inflammatory disease characterize by hyperproliferative keratinocytes. The causes of this disease are unclear, and many side effects are evident as a result of certain treatments of psoriasis. In this work, we aimed at studying the in vitro effect of each of these following Thai herbal extracts, Alpinia galanga L. (rhizome), Curcuma longa L. (rhizome) and Annona squamosa L.(leaf), containing anti-psoriatic activity on the expression of caspase 9, the protein known to play a critical role in the intrinsic apoptotic pathway. The HaCaT keratinocyte cell line was chosen as an in vitro model. Based on Western blot analysis, the extract derived from Annona squamosa L. leaf at all three concentrations tested significantly reduced the expression of procaspase 9 (P<0.05), and two higher concentrations, 0.5IC50 (3.15 µg/mL) and IC50 (6.3 µg/mL), of this herbal extract significantly increased the expression of caspase 9 protien (P<0.05). Confocal immunofluorescence microscopy subsequently confirmed this finding. Taken together, this might suggest that the ethanolic extract of Annona squamosa leaf with anti-psoriatic activity could exert its biological effect by activating the cleavage of rocaspase 9 into caspase 9, thus inducing the intrinsic apoptotic pathway.

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CD8

Kim et al._Fig. S1. A. Thymus. B. Gated on CD4 - CD8 - subset. PIDD +/+. PIDD -/-. CD44. CD8. CD4. CD25. C. BM. CD43. IgM. B220. D. E. Spleen. LN. counts. CD4. B220. CD8. Spleen. F. IgD. B220. IgM. Kim et al._Fig. S2. 1800 1600 1400 1200 1000 800 600 400

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Time (hour) 0 12 24 36 48

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Procasp.-9 mut + + + + - +

A. B. IP:C-9. IP:FLAG. TOTAL. IP:C-9. TOTAL. Procasp.-9 mut + + + + - + TUCAN-FLAG + + + + + - cyt.c/dATP + - + - - -. Procasp.-9 mut + + -

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Supplemental Figure 1

HuT102. *. Mock myr-AKT. - + - +. LBH589. Annexin-V positive cells (%). p-AKT. 60kDa. AKT. 60kDa. Caspase-3. 35kDa. - + - +. LBH589. cleaved casp-3. 19/17kDa. Mock myr-AKT. 47kDa. Caspase-9. HuT102. cleaved casp-9. 37kDa. Caspase-2. 51kDa. 32kDa.

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Intrinsic Apoptosis Pathway

The intrinsic apoptosis pathway is initiated by, for example, chemotherapy and/or radiotherapy. It is activated by a range of exogenous and endogenous stimuli, such as DNA damage, ischemia, and oxidative stress. Moreover, it plays an important function in development and in the elimination of damaged cells.

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