A. PUbx Gal4 > GFP. γ Ubx. B. C. 120. γ Ubx. γ GFP. 100. 100. 80. 80. γ GFP. A1. 60. wt. arm Gal4 > VCU. 60. % of arm expression level. % of wt A1 expression level. γ Ubx. 40. γ GFP. 40. T2. 20. 20. γ Ubx γ GFP. 0. 0. arm Gal4 > VCU (1). PUbx Gal4 > VCU (2). 2.By karlyn
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Simple regulatory systems:. The GAL4 Regulon. + Glucose (= no Galactose). no Glucose + Galactose. Gal3p. Gal3p. Gal4 binds to UAS G sites and regulates genes involved in Galactose metabolism. The dissociation model. The non-dissociation model. TBP. TBP.
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A. C. PabdA Gal4 >. en Gal4 > VCAHD VNEHD. γ GFP. 120. 18°C. 29°C. 100. BiFC. BiFC. 80. VCA. 60. % of VCA expression level. γ GFP. 40. γ AbdA. γ AbdA. 20. 0. VCAHD (1). 1. B. PabdA Gal4 >. γ HA. γ HA. γ HA. 120. 100. 80. VNE. merge. merge.
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