ChE 461/861. Introduction to Biochemical Engineering Instructor Dr. C. Niu Chemical Engineering. Research Area. Biosorption: Use biomaterials to adsorb inorganic (metals) or organic compounds from liquid phase Biomaterials: microorganism: Penicillium chrysogenum
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Recombinant DNA Technology. What is different between these 2 sequences? GGAATTCCTAGCAAT CCTTAAGGATCGTTA CTACGTGAGGAATTC GATGCACTCCTTAAG. Only order of bases!
Recombinant DNA Technology. Some basic terminology…. Recombinant DNA (rDNA)- contains DNA from 2 sources; DNA is inserted into that of another organism Vector- DNA piece that can be modified to accommodate a piece of foreign DNA
Recombinant DNA Technology………. BTEC3301. DNA Libraries. How do you identify the gene of interest and clone only the DNA sequence you are interested? Read Pg 63. DNA Libraries. Library screening of gene of interest
DNA Recombinant Technology. DNA recombinant. Genetic Engineering. The manipulation of an organism endowment by introducing or eliminating specific gene A gene of interest is inserted into another organism, enabling it to be cloned, and thus studied more effectively
Recombinant DNA Technology. Recombinant DNA Technology. T echnique that allows DNA to be combined from different sources DNA code universal Foundation of genetic engineering. Review. What are enzymes? What function do they serve? What makes up the DNA sequence?
Recombinant DNA Technology. A set of methods used to locate, analyze, alter, study, and recombine DNA sequences Recombinant DNA is DNA in which nucleotide sequences from two different sources (even different species) are combined in the laboratory to produce a new combination of genes.
Recombinant DNA Technology. Reference Books. Hortons’ Principles of Biochemistry 4th edition Molecular Biology , Robert Weaver 2nd edition Harpers’ Biochemistry 26th edition Styers’ Biochemistry. Objectives:
DNA recombinant technology. Definition of recombinant DNA technology( 基因重組 ). A series of procedures used to recombine DNA segments.. Under certain conditions, a recombinant DNA molecule can enter a cell and replicate. History of recombinant DNA technology.