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Biotechnology: Presant Status & biosafety concerns Department of Agriculture. H. M.J.Bandara Plant Quarantine Officer Sri Lanka . Department of Agriculture. Mandatory Crops: Cereals, legumes, condiments, fruit crops, root & tuber crops, oil seed crops vegetables, floricultural plants.

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biotechnology presant status biosafety concerns department of agriculture

Biotechnology: Presant Status & biosafety concernsDepartment of Agriculture

H. M.J.Bandara

Plant Quarantine Officer

Sri Lanka

slide2

Department of Agriculture

Mandatory Crops:Cereals, legumes, condiments, fruit crops, root & tuber crops, oil seed crops vegetables, floricultural plants

Activities:

Research & development, Seed certification & plant protection, Planting material production Distribution & Plant quarantine, Germplasm management, Botanic gardens, Agriculture extension, Education & training,

Finance & administration

tissue culture molecular techniques based on nuclear acid proteins
Tissue CultureMolecular techniques (Based on nuclear acid & proteins)

Biotechnology

Biotechnology is defined in many ways.

Tissue culture is considered one of the techniques. It’s associated directly with genetic transformation.

horticultural crop research and development institute tissue culture program

HORTICULTURAL CROP RESEARCH AND DEVELOPMENT INSTITUTE- Tissue Culture Program

Banana Tissue Culture

Papaya tissue culture

horticulture crop research and dvelopment institute

HORTICULTURE CROP RESEARCH AND DVELOPMENT INSTITUTE

Virus indexing (serological)

Banana

Papaya

Citrus

Microbiology

Food Research Unit

Fermentation Food analysis

plant virus indexing center

PLANT VIRUS INDEXING CENTER

Serology and PCR Based Virus Indexing- Banana Virus

Pineapple

RT PCR for Detection of RNA Virus

sitha eliya potato research center tissue culture program potato

SITHA ELIYA POTATO RESEARCH CENTER:Tissue Culture ProgramPotato

Straw berry

Pineapple

rardc bandarawela

RARDC, Bandarawela

Tissue Culture Program

Potato

Anthurium

Gerbera

Strawberry

Madonna lily

Virus indexing of potato by ELISA

rardc agunakolapelessa

RARDC, Agunakolapelessa

  • Thubakarwial

Banana

rice research and development institute rice biotechnology

RICE RESEARCH AND DEVELOPMENT INSTITUTE:Rice Biotechnology

Anther Culture

Exploitation of Somaclonal Variation

Wide Hybridization

national botanic gardens tissue culture program

NATIONAL BOTANIC GARDENS-Tissue Culture Program

Propergation of Endemic & rare Plants

Floriculture Plants

Mutation induction

plant genetic resource center tissue culture program

PLANT GENETIC RESOURCE CENTER: Tissue Culture Program

Banana

Citrus spp

  • In vitro pineapple potato Conservation
  • Papaya tissue Culture
plant genetic resource center tissue culture program13

PLANT GENETIC RESOURCE CENTER: Tissue Culture Program

Anther Culture in rice Haploid Culture

a good system for transformation

pgr rrdi rice research pcr based dna markers for breeding gene pyramiding

PGR/RRDI- Rice researchPCR based DNA markers for breeding/gene pyramiding

Gall midge resistance

  • Thrips resistance
  • Bactria leaf blight resistance
  • BPH resistance
slide15

Genetic Characterization by DNA markers

Mango - Mangifera zeylanica Magifera indica

Maize(PGRC/MI)

Finger printing of hybrids

Pathogens - Ralstonia, BLB

Onion - Characterization & breeding

future work

Future Work

SSR markers for rice

AFLP for fingerprinting of germplasm & Varieties

Northern blot techniques for

Analysis of HSP regular gene Expression

Isolating the fragment for Coat protection gene papaya Ring spot virus

application of tools in biosafety regular measures

Application of Tools in Biosafety Regular Measures

Tissue Culture Safe handling of materials Restricted multiplication

Serological methods Testing &detection of foreign

Genes bases in proteins

Serological method should not be the sole method.

Genes expression (transgenic)is spatially & temporally controlled

PCR/Blot methods Based on DNA More sensitive method.Detect only presence or foreign DNA

national plant quarantine service

National Plant Quarantine Service

Requirements

With the existing infrastructure & function and provided with additional strength (Trained staff, Detection equipment, Risk assessments Studuo) NPQS could undertake the regulatory activity

institutional biosafety committee
Institutional Biosafety Committee
  • Liaison with the national committees
  • Identification of institutes:transformation work,GMO detection /testing,regulation & risk assessment
  • Preparation of guidelines on biosafety regulations,risk assessments & management
  • Maintain constant access to relevant data base or registers both local & foreign
  • Monitoring & evaluation of programs
  • Building awareness
requirements for implementation of the biosafety procedures
Requirements for Implementation of the Biosafety Procedures
  • Policy level support
  • Financial,administrative & legislative support
  • Biosafety clearing house mechanism
  • Monitoring, evaluation & inspection services
  • Training of decision makers,&technical level scientists & technicians
  • Facility enhancement
  • Public awareness, education & promotion of public participation including stake holders.
genetic transformation of agricultural crops

GENETIC Transformation ofAgricultural Crops

Policy level decisions & if necessary, changes

Identification of priority problems/crops

Identification of Institutes

Facility development