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Biotechnology: Presant Status & biosafety concerns Department of Agriculture. H. M.J.Bandara Plant Quarantine Officer Sri Lanka . Department of Agriculture. Mandatory Crops: Cereals, legumes, condiments, fruit crops, root & tuber crops, oil seed crops vegetables, floricultural plants.
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Plant Quarantine Officer
Research & development, Seed certification & plant protection, Planting material production Distribution & Plant quarantine, Germplasm management, Botanic gardens, Agriculture extension, Education & training,
Finance & administration
Biotechnology is defined in many ways.
Tissue culture is considered one of the techniques. It’s associated directly with genetic transformation.
Banana Tissue Culture
Papaya tissue culture
Virus indexing (serological)
Food Research Unit
Fermentation Food analysis
Serology and PCR Based Virus Indexing- Banana Virus
RT PCR for Detection of RNA Virus
Tissue Culture Program
Virus indexing of potato by ELISA
Exploitation of Somaclonal Variation
Propergation of Endemic & rare Plants
Anther Culture in rice Haploid Culture
a good system for transformation
Gall midge resistance
Mango - Mangifera zeylanica Magifera indica
Finger printing of hybrids
Pathogens - Ralstonia, BLB
Onion - Characterization & breeding
SSR markers for rice
AFLP for fingerprinting of germplasm & Varieties
Northern blot techniques for
Analysis of HSP regular gene Expression
Isolating the fragment for Coat protection gene papaya Ring spot virus
Tissue Culture Safe handling of materials Restricted multiplication
Serological methods Testing &detection of foreign
Genes bases in proteins
Serological method should not be the sole method.
Genes expression (transgenic)is spatially & temporally controlled
PCR/Blot methods Based on DNA More sensitive method.Detect only presence or foreign DNA
With the existing infrastructure & function and provided with additional strength (Trained staff, Detection equipment, Risk assessments Studuo) NPQS could undertake the regulatory activity
Policy level decisions & if necessary, changes
Identification of priority problems/crops
Identification of Institutes