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Investigating CD44 Expression in Tumor Invasion and Migration

This study explores the role of CD44 in cancer invasion and migration through gene expression analysis and cell behavior assays on MKN-45 and MKN-74 cell lines. The research examines the impact of CD44 levels on tumor volume, mRNA expression levels, migration, and spheroid formation.

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Investigating CD44 Expression in Tumor Invasion and Migration

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  1. control + 7.13 + 7.13/CD44low + 7.13/CD44high MKN45 MKN45 MKN45 MKN45 MKN74 MKN74 MKN74 MKN74 # * 4000 10 8 MKN45 MKN45 9 3500 7 MKN74 MKN74 8 # 3000 6 7 * 2500 * 5 6 Fold change in invasion Tumors volume in mice (mm3) 2000 5 4 4 # 1500 3 3 1000 2 2 500 1 1 0 0 0 Ctrl + 7.13 CD44low CD44high Ctrl + 7.13 CD44high CD44low Ctrl + 7.13 CD44low CD44high Ctrl + 7.13 20 CD44 * Snail1 18 Vimentin * 16 ZEB1 14 * 12 10 Relative mRNA level * 8 * * * 6 * 4 2 0 Ctrl + 7.13 Ctrl + 7.13 MKN-45 MKN-74 A C D B * 30 3,5 * * * * 3 25 # 2,5 20 * 2 Fold change in migration 15 Relative expression level of CD44 Fold induction of elongated cells * * 1,5 # 10 1 5 0,5 0 0 Ctrl + 7.13 CD44low CD44high Ctrl + 7.13 Ctrl Ctrl + 7.13 + 7.13 E F G * 70 * * 60 50 40 Number of spheroids # 30 20 * 10 0 Ctrl + 7.13 CD44low CD44high Ctrl + 7.13 H

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