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DASL™ Assay for Monitoring Gene Expression in cDNA with Oligonucleotide Targeting

The DASL™ (cDNA-mediated annealing, selection, extension, and ligation) assay is a powerful technique for monitoring gene expression by targeting specific sequences in cDNAs using sets of query oligonucleotides composed of multiple parts. This method provides insights into gene activity in formalin-fixed, paraffin-embedded tissues, which is particularly valuable in breast cancer research. The study by Paik et al. (2005) highlights the application of this molecular technique, advancing our understanding of gene expression in clinical samples.

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DASL™ Assay for Monitoring Gene Expression in cDNA with Oligonucleotide Targeting

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  1. Figure 7 The cDNA-mediated annealing, selection, extension and ligation (DASL™) assay monitors gene expression by targeting sequences in cDNAs with sets of query oligonucleotides, which are composed of multiple parts Paik S et al. (2005) Technology Insight: application of molecular techniques to formalin-fixed paraffin-embedded tissues from breast cancer Nat Clin Pract Oncol 2: 246–254 10.1038/ncponc0171

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