AFLP Amplified Fragment Length Polymorphism. AFLP. - a method based on PCR developed in 1995 by Vos et al. Involves the use of RFLP and PCR techniques Compared with the widely used RFLP, AFLP is faster, less labour intensive and provide more information.
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The AFLP technique is based on the principle of selectively amplifying a subset of restriction fragments from a complex mixture of DNA fragments obtained after digestion of genomic DNA with restriction endonucleases.
Procedures in AFLP:
- Adaptor Ligation
Two different restriction endonucleases are used in digestion. One is 4-base cutter (MseI) and the other one is 6-base cutter (EcoRI).