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GENE TRANSFER AND GENETIC ENGINEERING PowerPoint Presentation
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GENE TRANSFER AND GENETIC ENGINEERING

GENE TRANSFER AND GENETIC ENGINEERING

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GENE TRANSFER AND GENETIC ENGINEERING

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  1. GENE TRANSFER AND GENETIC ENGINEERING CHAPTER 8

  2. Gene Transfer • Vertical to offspring • Lateral (horizontal) to others in same generation

  3. Bacterial Gene Transfer and Recombinant DNA Technology • Transformation • Griffith’s experiment

  4. Transformation • Mechanism • Significance Recombination?

  5. Transduction • Mechanism • Significance Transducing phages: p1, p22 Can be used in genetic mapping http://jb.asm.org/cgi/reprint/174/23/7876

  6. Conjugation • Mechanism • Significance Conjugation pilus Also used for mapping

  7. Plasmids • F plasmid (conjugation) • Resistance plasmids • Virulence plasmids

  8. Plasmids (cont.) • Tumor-inducing plasmids • Agrobacterium tumefaciens and plants • Plasmids conferring novel catabolism Degradation pathways on a plasmid

  9. Genetic Engineering • Genetic fusions • reporter fusions • promoter fusions

  10. Genetic Engineering • Protoplast fusion • electroporation

  11. Genetic Engineering (cont.) • Gene Amplification • Recombinant DNA cloning

  12. Applications • Medical • Industrial • Agricultural • Hybridomas

  13. Risks and Benefits Frankenstein? Frankenfoods?

  14. Recombinant DNA technology • Plasmids- autonomous replicating extrachromasomal DNA • restriction enzymes

  15. Viral technology- phage cloning

  16. Homologous recombination Used to make gene knockouts in bacteria and yeast

  17. Transposons - jumping genes

  18. Evidence for extensive transfer of antibiotic resistance genes inBacteroides sp. http://mcb.illinois.edu/faculty/profile/abigails